庆大霉素与速尿联合静脉注射对听觉脑干诱发电位及耳蜗琥珀酸脱氢酶的影响  

The Effect of Single Combined Administration of Gentamycin and Furosemide on Auditory Brainstem Responses and Succinate Dehydrogenase Activity in Guinea Pigs

作　　者：刘海瑛[1] 高文元[1] 文文[1] 张琰敏[1] 

机构地区：[1]第二军医大学基础部生理学教研室听觉研究室,上海200433

出　　处：《中国眼耳鼻喉科杂志》2006年第1期12-14,17,i0002,共5页Chinese Journal of Ophthalmology and Otorhinolaryngology

基　　金：国家自然科学基金(30470419)

摘　　要：目的观察庆大霉素(gentamycin,G)及速尿(furosemide,F)联合一次性静脉注射对豚鼠听觉脑干诱发电位(auditory brainstem responses,ABR)及耳蜗琥珀酸脱氢酶(succinate dehydrogenase,SDH)的影响。方法杂色豚鼠随机分为3个对照组:①生理盐水(N 组),②F100 mg/kg,③G100 mg/kg;3个实验组:①G100 mg/kg+F80mg/kg,②G100 mg/kg+F90 mg/kg,③G100mg/kg+F100mg/kg。各组均一次性静脉给药,并分别于给药前及给药后1、2、3、7、14、21 d 测试 click 及4、8 kHz 的 ABR 阈值,计算阈移。最后一次测试后,各组取耳蜗,检测 SDH 活性并计数毛细胞,计算毛细胞缺失率。结果 G100、F100组与 N 组给药后的 ABR 阈值与给药前相比均无明显改变;G100+F80组给药后 ABR 阈值与 N 组比较无显著差异;G100+F90组与 G100+F100组给药后3d 阈移达到最大,与 G100+F80组比较均有显著差异;给药后7d 至21d,两组 ABR 平均阈移均未出现明显改变。SDH 检测及基底膜铺片结果显示 G100及 F100组的结果与 N 组基本一致;G100+F80组仅有散在的 SDH 染色变浅甚至消失的外毛细胞;G100+F90及 G100+F100组以底回外毛细胞(outer hair cell,OHC)受损及缺失显著,SDH 变化显著,各组内毛细胞均无明显受损。结论庆大霉素与速尿联合一次大剂量静脉注射能够使豚鼠产生 ABR 永久阈移,对耳蜗 SDH 活性的影响是机制之一。Purpose To explore the effect of single combined administration of gentamycin（G） and furosemide（F） on auditory brainstem responsese （ABR） and succinate dehydrogenase（SDH） acitivity of cochlea in guinea pigs, Methods Pigmented guinea pigs were randomly divided into G100 mg/kg ＋ F80 mg/kg; G100 mg/kg ＋ G90 mg/kg; G100 mg/kg ＋ F100 mg/kg; G100 mg/kg; F100 mg/kg and saline group. All drugs were administered intravenously for only a single dose. ABR were measured beofre treatment and 1,2,3,7,14,21 d post-treatment respectively. After the final measurement of ABR, the cochlea were removecd for SDH detection and surface preparation. Results Animals receiving a single dose of G or F showed httle changes of ABR thresholds as well as SDH activity. For G100 ＋ F80, the ABR were also not significantly different from saline group. However, the threshold shifts of G100 ＋ F90 and G100 ＋ F100 group were all elevated after treatment,with the most changes at 3 d post-treatment, significantly higher than G100 ＋ F80 group. Moreover, almost no decline of ABR occured between 3 d and 21 d post-treatment. Obvious decrease in SDH acitivity and outer hair cell loss were observed in both G100 ＋ F90 and G100 ＋ F100 groups, with more obvious changes in the basal turns and G100 ＋ F100 groups. Inner hair ceils were almost kept intact in all groups. Conclusion The combined single administration of large dose of G and F may induce persistent ABR threshold shifts in guinea pigs and the changes in SDH activity may be one of the mechanisms.

关 键 词：庆大霉素 速尿 听觉脑干诱发电位 琥珀酸脱氢酶 耳蜗 豚鼠 

分 类 号：R96[医药卫生—药理学]