大骨节病患者血清促炎症细胞因子水平的检测  

Detection of proinflammatory cytokine levels in sera from patients with Kaschin-Beck disease

作　　者：朱参胜[1,2] 冯清华[3] 于曰祥[3] 王东琳[1] 陈明[3] 王晓云[3] 金伯泉[1] 

机构地区：[1]第四军医大学基础部免疫学教研室 [2]陕西省地方病防治研究所,陕西西安710003 [3]陕西省地方病防治研究所

出　　处：《细胞与分子免疫学杂志》2006年第1期84-85,共2页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的探讨前炎症细胞因子TNF、IL-1β和IL-6在大骨节病(KBD)发病机制中的作用。方法采集62例KBD患者和60例健康对照的血清标本,采用双抗体夹心ELISA法测定血清前炎症细胞因子TNF、IL-1β和IL-6的水平。结果KBD患者血清IL-1β和IL-6的水平分别为(238.4±698.5)ng/L和(164.4±661.4)ng/L,健康人分别为(74.5±130.0)ng/L和(52.2±154.6)ng/L,但它们之间差异均不显著(P>0.05)。然而,KBD患者血清TNF的水平[(109.2±145.3)ng/L]高于健康对照[(40.9±89.7)ng/L],差异非常显著(P<0.01)。患者血清TNF与IL-1β的水平及血清TNF与IL-6水平的相关性均不显著(r值分别为0.0387和0.2135,P>0.05)。血清IL-1β与IL-6的水平呈显著的正相关(r=0.3460,P<0.01)。结论血清前炎症细胞因子水平的升高,可能与大骨节病的发病有关。AIM. To explore the role of proinflammatory cytokines （TNF, IL-1β and IL-6） in the pathogenesis of Kaschin-Beck disease （KBD）. METHODS： The levels of serum TNF, IL-1βand IL-6 from 62 KBD patients and 60 healthy persons were detected by double antibody sandwich ELISA. RESULTS： The levels of serum IL-1β and IL-6 in KBD patients were （238.4 ±698.5） ng/L and （ 164.4 ±661.4） ng/L, respectively, but they were higher than those in healthy persons, being （74.5 ± 130.0） ng/L and （52.2±154.6） ng/L, respectively. There were no significant differences between them （ P 〉 0.05）. However, the level of ser- um TNF in KBD patients [ （ 109.2±145.3 ） ng/L] was significantly higher than that in healthy persons [ （40.9± 89.7 ） ng/L] （P〈0. 01 ）. Moreover, there was no correlation between serum TNF and IL-1β levels （ r = 0. 0387, P 〉 0.05 ） and TNF and IL-6 in KBD patients （ r=0. 2135, P〉0.05）, but there was positive correlation between serum IL-1β and IL-6 levels （ r=0.346, P〈0.01 ）. CONCLUSION： The elevated proinflammatory cytokine levels in sera may relate to the pathogenesis of Kaschin-Beck disease.

关 键 词：大骨节病 TNF IL-1 β IL-6 

分 类 号：R593.22[医药卫生—内科学]