23-羟基桦木酸体外抑制血管生成的实验研究  

In vitro inhibitory effect of 23-HBA on angiogenesis

作　　者：蒋孟军[1] 杨敏[1] 周尧远[1] 张荣军[1] 曹国宪[1] 蔡刚明[1] 王广基[1] 

机构地区：[1]江苏省原子医学研究所核医学国家重点实验室,江苏无锡214063

出　　处：《细胞与分子免疫学杂志》2006年第1期88-91,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：江苏省自然基金资助项目(No.BK2004025)

摘　　要：目的探讨23-羟基桦木酸(23-HBA)对体外血管生成的抑制作用。方法采用磺酰罗丹明B(SRB)法测定23-HBA对人毛细血管内皮细胞(HMEC)增殖、迁移和小管形成的影响;用免疫组化染色法检测HMEC中CD31表达的变化。结果23-HBA体外可明显抑制HMEC增殖(IC50为40.44mg/L)、迁移和小管的形成,且呈明显的剂量依赖性。23-HBA的浓度为10mg/L时,HMEC中CD31的表达明显降低。结论23-HBA体外具有明显抑制血管生成的作用,有可能成为有效的血管生成抑制剂。AIM： To study the inhibitory effect of 23- HBA on angiogenesis in vitro. METHODS ： The effect of 23- hydroxy butulinic acid （23-HBA） on the in vitro proliferation of human microcapillary endothelial cells （HMECs） was examined by sulfonylrhodamine B （SRB） assay. The effect of 23-HBA on endothelial cell migration, and tubule formation on Matrigel was also observed. The CD31 expression in HMECs was dectected by immunohistochemical staining. RESULTS： The proliferation of HMECs was inhibited significantly by 23-HBA with IC50 being 40.44 mg/L. 23-HBA inhibited endothelial cell migration and tubule formation in a dose-dependent manner. The expression of CD31 in HMECs was reduced after treatment with 10 mg/L 23-HBA. CONCLUSION： 23-HBA can inhibit angiogenesis in vitro, which would become a promising antiangiogenic drug.

关 键 词：23-HBA 血管生成 人毛细血管内皮细胞 

分 类 号：R979.1[医药卫生—药品]