丝状支原体丝状亚种SC型中国分离株分子流行特征  

Molecular characteristic of Mycoplasma mycoides subsp. Mycoides small- colony type (MmmSC) for China strains

作　　者：辛九庆[1] 李媛[1] 高玉龙[1] 王砚范[1] 钱爱东[2] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所国家牛传染性胸膜肺炎参考实验室黑龙江,哈尔滨150001 [2]吉林农业大学动物科技学院,吉林长春130118

出　　处：《中国预防兽医学报》2006年第1期38-40,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家"十五"科技攻关重大专项基金项目(2002BA518A04)

摘　　要：收集了6个来自不同地方中国历史流行的丝状支原体丝状亚种SC型(Mycoplasma mycoidessubsp.MycoidesSC,MmmSC)毒株与PG1、Y-goat进行分子特征比较。全菌体蛋白电泳与PG1完全相同,而与Y-goat不同;PCR结果显示6个中国分离株与PG1、Y-goat都没有缺失8.84 kb片段;LppB全基因序列比较发现与PG1同源性达99%,而与Y-goat同源性很低;多位点基因序列类型(Multilocus sequence types,MST)分析显示与非洲澳大利亚群完全相同。以上证据显示中国CBPP流行株应归属于非洲澳大利亚群。Contagious bovine pleuropneumonia （CBPP） is a bacterial disease by Mycoplasma mycoides subsp. Mycoides SC （MmmSC） included in list A of the Office International Epizooties .The comparison of Molecular characteristic between 6 China strains from different area and PG1,Y-goat. SDS-PAGE of cells is as same as PG1, but different from Y-goat. PCR result shows 6 China strains and PG1 and Y-goat are not lack of the 8.84kb genomic segment .The compare with gene of LppB show 99% homology for PG1, but lower homology for Y-goat. MST analysis indicate that it is the same between 6 China strains and African/Australian group. That evidence confirms 6 China strains of CBPP belone to African and Australia cluster.

关 键 词：MmmSC PG1 特征 

分 类 号：S852.659.5[农业科学—基础兽医学]