志贺氏菌对第三代头孢菌素的耐药机制初步研究  

The preparatory study on the mechanism of S. flexneri resistance to the third generation cephalosporins

作　　者：梁军[1] 胡功政[1] 苑丽[1] 司红彬[1] 杨玉荣[1] 匡秀华[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国预防兽医学报》2006年第1期58-62,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助项目部分内容(30471307)

摘　　要：通过对福氏志贺氏菌的诱导及其诱导耐药菌MIC与β-内酰胺酶、超广谱β-内酰胺酶(ESBLs)的检测,探讨福氏志贺氏菌对三代头孢菌素的耐药机制。结果表明,随诱导代数的增加,诱导菌对抗菌药的MIC值亦逐步增大,诱导至30代时,三代头孢对诱导菌的抗菌活性显著降低,MIC范围为2μg/mL￣32μg/mL。当对福氏志贺氏菌诱导5代时,已有β-内酰胺酶的产生;诱导至15代时,已有ESBLs产生。诱导至30代时,ESBLs已大量产生。志贺氏菌对三代头孢菌素的主要耐药机制为超广谱β-内酰胺酶的产生。Researching the mechanism of S.flexneri resistance to the third generation cephalosporins by way of inducing the S. flexneri,determining their minimal inhibitory concentration to some antibiotics and determining the β-lactamase（including ESBLs） that they produces. The result showed that the value of MIC was increasing as the inducing was enhanced, when the S. flexneri was induced to 30th generations , the antibiotic activities of the third generation Cephalosporins to them decreased sharply, with the value of MIC from 2 μ g/mL to 32 μ g/mL. Induced to 5th generations,the β-lactamase could be determined and 15th generations the ESBLs could be determined also. To 30th generations,the S.flexneri had produced a lot of ESBLs.The main machanism of drug resistance to the third generation cephalosporins of S.flexneri is the ESBLs-producing.

关 键 词：志贺氏菌 头孢菌素 耐药机制 Β-内酰胺酶 超广谱Β-内酰胺酶 

分 类 号：S852.61[农业科学—基础兽医学]