魏氏梭菌类毒素疫苗研制及其免疫家兔抗体消长规律  

Producing research of the C.perfringens type A toxoid vaccine

作　　者：柴同杰[1] 刘文波[2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]南京农业大学动物医学院,江苏南京210095

出　　处：《中国预防兽医学报》2006年第1期101-104,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：1999年山东省重点攻关项目;鲁科技[1999]第431号鲁财工指字[1999]和86号

摘　　要：本研究分别从山东省的莱芜、泰安、潍坊的三个养兔场发生疑似梭菌性下痢的病兔体分离到三株魏氏梭菌,鉴定为A型。利用该分离菌株和A型魏氏梭菌标准株(CVCC37)所产外毒素经甲醛灭活并加入氢氧化铝胶佐剂吸附浓缩后制备了A型魏氏梭菌类毒素疫苗。对该疫苗分别进行了安全性检验、有效免疫剂量试验和接种家兔1～23周血清中抗毒(素Ab)消长规律研究。结果表明,魏氏梭菌外毒素用0.3%甲醛32 h能够彻底灭活,并最大可能地保持其抗原性;制备的魏氏梭菌类毒素疫苗无毒副作用,安全可靠;用野毒株和标准株制备的类毒素疫苗对家兔的有效免疫剂量为2 mL/只,免疫保护效果可靠;采用野毒株和标准株制备的类毒素疫苗接种家兔后第二周血清抗毒素效价迅速升高,到第四周达最高峰,分别为6.6-和7.125 log2,较高抗体滴度维持约17周后缓慢下降,至23周时血清平均抗毒素滴度仍维持在4.0-和3.4 log2以上,所以,该类毒素疫苗的免疫保护期可以设定为6个月。With C.perfringens field strain and standard strain,the toxin was produced and was inactivated by 0.3 % formalin.Then it was mixed with 5 % AI（OH）3 and the C.perfringens toxoid vaccine was successfully produced. In order to test the immune protective period of the vaccine serum antibody＇s growth and decline regulation of the rabbits was detected,which was immunized with the toxoid vaccine.From 7th day,the serum antitoxin was detected and antitoxin titer were very low （1.125-and 0.8 log2 respectively）.since the second week the antitoxin concentration soon increased and amount to 4.875-and 4.11 logz respectively.It was reached highest titer with 7.125 log2 for std.and 6.6 log2 for field strain in 30 days post immunization. The high level antitoxin could be maintained for about 3 months. In the 23rd week, the antitoxin titer was still preserved 4.0-and 3.4 log2 respectively. By the t-test, there was no remarkable difference （P 〉 0.05）between the antitoxin＇s levels of two group rabbits. The results showed that this vaccine had high immunity effect. The immune protective period of the CI.perfringens toxoid vaccine can arrive for 6 months.

关 键 词：A型魏氏梭菌 类毒素疫苗 抗毒素 抗体消长规律 家兔 

分 类 号：S852.61[农业科学—基础兽医学]