慢性毒鼠强中毒后大鼠脑细胞色素C表达的实验研究  

Expression of Cyt C in Brain of Rats Poisoned by Tetramine

作　　者：李斌[1] 陈伟杰 闫红涛[1] 吴敬杰[1] 金波[1] 廖志钢[1] 

机构地区：[1]四川大学华西基础医学与法医学院法医病理学教研室,成都610041 [2]浙江省余姚市公安局刑侦大队

出　　处：《四川大学学报（医学版）》2006年第1期27-29,共3页Journal of Sichuan University(Medical Sciences)

基　　金：教育部高等学校博士学科点专项科研基金(编号20030610010);四川大学基金资助

摘　　要：目的探讨大鼠慢性毒鼠强中毒后脑部细胞色素C(CYT C)的表达情况,为毒鼠强中毒案件的法医学鉴定提供新的指标。方法建立大鼠毒鼠强慢性中毒模型,用免疫组化与图像分析技术分别检测中毒后不同时间大鼠脑皮质、海马CYT C的表达。结果对照组大鼠脑内CYT C有微量表达,实验组大鼠中毒后30MIN CYT C表达开始增强,24H到达高峰,3D后开始下降,10D后基本恢复正常。结论CYT C表达的规律性变化可望成为慢性毒鼠强中毒的法医学鉴定依据,并可为临床推断慢性毒鼠强中毒的不同时期提供证据。Objective To investigate the evidence for the judgement on legal cases of intoxation by tetramine. Methods Fifty rats given tetramine by lavage were sacrificed at different time after intoxation. The expression of Cyt C was detected by immunohistochemistry, and the reasults were assessed by an image analysis system. Results There was a slight expression of Cyt C in control group. In intoxation group the expression of Cyt C increased after the rats were given tetramine for 30 minutes; it reached the peak at 24 hours after intoxation and kept up the level for 3 days, then it began to decrease and returned to the control level in 10 days. Conclusion The regular change of Cyt C expression might be a useful sign for assessment in legal cases of intoxation by tetramine.

关 键 词：毒鼠强 细胞色素C 免疫组织化学 

分 类 号：D919.4[医药卫生—法医学]