5个STR基因座遗传多态性研究及复合扩增试剂盒的构建及应用  

Analysis of the Multi-amplified 5 STR Loci and Their Allelic Distribution in Both Han Populations in Chengdu City and Yunnan Province

作　　者：辛军国[1] 梁伟波[1] 吕梅励[1] 杜宏[1] 陈国弟[1] 张林[1] 

机构地区：[1]四川大学华西基础医学与法医学院教育部人类疾病生物治疗重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2006年第1期84-87,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30171033);教育部科学研究重点项目(01143);四川省青年基金(2001-2)资助

摘　　要：目的用分子克隆技术制备出D7S820、D13S317、D5S818、D3S1358等四个STR基因座和AMELO-GENIN基因座的分型标准物,并建立用复合PCR进行同步扩增检测的方法,以提高检测效率,降低成本。方法用复合扩增方法检测130名云南省汉族和130名成都汉族无关个体的5个基因座的等位基因分布,五个基因座的各等位基因互不重叠,PCR复合扩增产物电泳分型清晰,与单基因座检测结果一致。结果D7S820、D13S317、D5S818、D3S1358四个STR基因座在两个汉族群体中的基因型分布均符合HARDY-WEINBERG平衡,在云南省汉族中分别发现7、7、8和8个等位基因,在成都汉族中分别发现8、7、8和7个等位基因。四个STR基因座的等位基因频率的分布在两个群体之间差异无统计学意义。结论五个基因座的累计识别能力和非父排除率较高,用自制的STR分型标准物,银染法检测这五个基因座的复合扩增在法科学领域中有较高的实用价值。Objective To illuminate the multi-amplified 5 STR loci and their allelic distribution in Hans by means of STR-DNA typing with improved efficiency and decreased cost. Methods We have established an allelic ladder of D7S820, D13S317, D5S818, D3S1358 and Amelogenin loci via the cloning techniques. With this home- made allelic ladder, we established successfully a multiplexing polymerase chain reaction （PCR） method, followed by denaturing polyacrylamide gel electrophoresis （PAGE） and silver staining. DNA samples collected from 130 unrelated Han individuals in Yunnan and Chengdu were analyzed. The non-overlapping of the allelic fragments of the five loci allowed the detection to be accomplished successfully. Results No difference of the ge,lotyping results of the single locus amplification and multiplexing was observed. The genotype distributions of 4 STR were in accordance with the Hardy-Weinberg equilibrium. 7, 7, 8 and 8 alleles of D7S820, D13S317, DSS818, and D3S13S8 loci were observed in Yunnan Han population, as well as 8, 7, 8 and 7 alleles in Chengdu Han population respectively. No significant difference in the allele distribution of these loci was seen between these two Han populations. Conclusion This multiplexing system with home-made allelic ladder has a high combined discrimination power and exclusion power. It is a valuable tool in forensic science practice.

关 键 词：STR基因座 复合PCR 基因频率分布 

分 类 号：R346[医药卫生—基础医学]