N-copine基因反义核酸对原代培养的小鼠皮质神经元的影响  

Effect of N-copine antisense oligonucleotide on primary cultured cortical neuron in mice

作　　者：唐元家[1] 邹洪[1] 谢青莲[1] 金玫蕾[1] 

机构地区：[1]中国科学院上海生命科学研究院,中国科学院研究生院上海200031

出　　处：《中国病理生理杂志》2006年第1期80-83,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30270219)

摘　　要：目的:通过研究N-copine基因反义核酸(antisense)对体外培养的小鼠大脑皮质神经元的影响,在细胞学层面上探索N-copine基因的功能。方法:原代培养小鼠大脑皮质神经元,用反义核酸抑制N-copine基因的表达,分析抑制N-copine基因表达对神经元生长及死亡的影响。结果:寡核苷酸处理72h,antisense组神经元轴突长度短了而且胞体变小,而对照组神经元轴突和胞体都正常生长;antisense组台盼蓝着色细胞明显多于对照组,培养液中LDH活力明显高于对照组。结论:抑制N-copine基因表达,能影响体外培养的皮质神经元的生长,并导致神经元严重损伤和死亡。这提示N-copine可能是神经元的一种保护性蛋白,可能具有防止神经元退变和促进再生的重要作用。AIM： To study the function of N - copine on cell level by investigating the effect of N - copine antisense on the cultured cortical neuron of mouse embryos. METHOIDS： Neuronal cultures were prepared from cerebral cortices of 16 - day old mouse （BALB/C） embryos. N - copine expression was blocked by using antisense oligonucleotides. The effect of inhibition of N - copine expression on the cultured neuronal viability and development was observed. RESULTS： With oligonucleotides treatment for 72 h, the length of neuronal axon and the area of neurosoma in antisense group were decreased markedly as compared with those in control group. The number of trypan - blue - stained neurons and the LDH activity of the culture media were increased significantly as compared with those in control groups. CONCLUSIONS： Inhibition of N - copine expression affects the development of cortical neuron cultured /n vitro, and induces the neuron demaged severely. The results suggest that N - copine is a functional protein in neurons, and it may play an important role in the regeneration of nervous system and preventing neuronal degeneration.

关 键 词：基因 N—copine 寡核苷酸类 神经元 反义核酸 小鼠 

分 类 号：Q2-33[生物学—细胞生物学] Q527