小鼠肾近曲小管上皮细胞顶侧膜钾通道的膜片钳研究  

Study of K^(+) channels at the apical membrane of mouse renal proximal tubule cell with patch-clamp

作　　者：刘亚利[1] 黄维[1] 高恩泉[1] 叶本兰[1] 

机构地区：[1]第三军医大学基础医学部生理学教研室,重庆400038

出　　处：《第三军医大学学报》2006年第1期44-46,共3页Journal of Third Military Medical University

摘　　要：目的研究小鼠肾脏近曲小管上皮细胞顶侧膜K+通道的生理学特性。方法采用膜片钳细胞贴附式单通道记录急性分离的近曲肾小管上皮细胞顶侧膜K+通道电流。结果该通道在超极化状态下表现为内向电流,斜率电导为(11.4±0.6)pS,且通道开放活动随极化程度增大而增加。在反极化状态下表现为外向电流,并具有内向整流特性。结论小鼠肾脏近曲小管上皮细胞顶侧膜上存在一类低电导的K+通道,其分布广泛,可能参与钾离子转运、调节细胞体积及维持电势能的稳定。Objective To study the property of the K ^＋ channels at the apical membrane of mouse renal proximal tubule cells. Methods Single-channel recording of the K^＋ channel currents in apical membrane of freshly isolated renal proximal tubule cells was performed using the cell-attached mode of the patch-clamp technique. Results This kind of potassium channel appeared to be an inward rectifier, and its limiting inward slope conductance was （ 11.4 ± 0. 6） pS. The channel activity increased with hyperpolarization. Conclusion The potassium channel with spontaneous current was observed in most of the cell-attached patches, which is thought to be involved in volume regulation, potassium recycling and stabilization of the apical potential.

关 键 词：肾脏近曲小管 钾离子通道 膜片钳 

分 类 号：R322.61[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]