铝敏化紫外分光光度法测定氧氟沙星  

The Determination of Ofloxacin by the Aluminium Sensitized UV-Spectrophotometric Method

作　　者：陶玉龙[1] 敖登高娃[1] 刘惠民[2] 阿娟[2] 

机构地区：[1]内蒙古大学化学化工学院,呼和浩特010021 [2]内蒙古农业大学理学院,呼和浩特010019

出　　处：《内蒙古大学学报（自然科学版）》2006年第1期23-25,共3页Journal of Inner Mongolia University：Natural Science Edition

基　　金：内蒙古教育厅项目(NJ03115)

摘　　要：研究了金属离子对氧氟沙星紫外光谱的影响及反应条件．结果表明，在pH3．5～4．9的NaAc—HAc缓冲溶液中，铝离子对氧氟沙星吸光度有显著增敏作用．Al—OFLX体系的测定波长λmax=292nm，线性范围为0．2～38．7μg／mL；回归方程为Y=0．0069＋0．09438C（C=μg／mL），r=0．9998；检出限为3．5×10^-2μg／mL．据此建立了测定药物制剂中氧氟沙星含量的铝敏化紫外分光光度法．方法灵敏、操作简便，用于药物制剂中氧氟沙星的分析测定，回收率在95％～105％之间，结果令人满意．The ultraviolet spectrophotometric characteristics of ofloxacin in the presence of Al（ Ⅲ ） and the condition of the reaction of ofloxacin with Al（ Ⅲ ） were studied. The experiments indicated that in pH 3. 5～4. 9 NaAc-HAc medium,Al（ Ⅲ ） could greatly enhance the absorption intensity of ofloxacin. The maximum absorption wavelength was 292 nm,and the linear range was 0. 2～38. 7μg/mL for ofloxacin. The regression equation was Y= 0. 0069＋ 0.09438C （C=μg/ mL, r=0. 9998） with the detection limit of 3. 5 × 10^-2μg/mL. This method has been successfully applied to the determination of ofloxacin in tablets.

关 键 词：氧氟沙星 铝 紫外分光光度法 药物制剂 

分 类 号：O657.32[理学—分析化学]