纳米羟基磷灰石/聚酰胺骨水泥增强骨质疏松松质骨性能的生物力学分析  

Biomechanical evaluation of augmentation of osteoporotic cancellous bone with injectable nano-hydroxyapatite/ polyamide cement

作　　者：黄伟[1] 安洪[1] 蒋电明[1] 李玉宝[2] 魏杰[2] 秦廷武[3] 

机构地区：[1]重庆医科大学附属第一医院骨科,重庆市400016 [2]四川大学纳米生物材料研究中心,四川省成都市610064 [3]四川大学生物治疗国家重点实验室/细胞与组织学工程研究室,四川省成都市610041

出　　处：《中国临床康复》2006年第1期53-56,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家"八六三"计划资助项目(2002AA326020)~~

摘　　要：目的:评价新型注射型纳米羟基磷灰石/酰胺66复合骨水泥体外增强聚骨质疏松性松质骨的生物力学性能,从而选择适合临床使用的骨水泥。方法:实验于2001-01/2003-01在重庆医科大学、四川大学纳米生物材料研究中心和四川大学生物治疗国家重点实验室完成。选取5具老年和1具青年的T1-T6脊柱和双侧股骨髁标本,经X线摄片未见明显病理性缺损和破坏。椎体压缩实验:将脊柱标本去除椎间盘和椎体后方成分后仅保留椎体34个。①老年椎体28个,随机分为4组:纳米羟基磷灰石/酰胺66含量60%的复合骨水泥组(HP1组)、纳米羟基磷灰石/聚聚聚酰胺酰胺66含量70%的复合骨水泥组(HP2组)、纳米羟基磷灰石/66含量80%的复合骨水泥组(HP3组)、骨质疏松组,7个标本/;青年组椎体6个,作为正常对照组。②HP1,HP2,HP3组分别注射含量为60%,70%和80%的纳米羟基磷灰石/酰胺66复合骨水泥5mL,骨质疏松聚组和正常对照组仅作穿刺。③测定每个标本的载荷-位移数据和压力-位移曲线,采样频率为10Hz。股骨髁松质骨扭转实验:用于实验的股骨髁10个。①老年股骨髁8个,随机分为4组:HP1,HP2HP3组及骨质,疏松组,2个/;青年股骨髁2个,作为正常对照组。②HP1,HP2,HP3组组分别注射含量为60%,70%和80%的纳米羟基磷灰石/酰胺66复聚合骨水泥20mL,骨质疏松组和正常对照组仅作穿刺。然后将各组股骨髁的松质骨制成10mm×10mm×30mm的松质骨条标本,5个/每组。测定各组的抗扭强度和抗扭刚度。结果:椎体压缩实验中保留34个椎体,股骨髁松质骨扭转实验中选用10个股骨髁,全部进入结果分析。椎体压缩实验:①各组屈服强度和最大抗压强度的测定:与骨质疏松组比较,HP1,HP2,HP3组均明显提高,HP1,HP2组尤为显著(P均<0.05);但HP1,HP2,HP3组仍均显著低于正常对照组(P<0.05)。②各组抗压刚度的测定:与骨质疏松组比较,HP1,HP2,HP3组均明显提高,HP1,HP2�AIM： To investigate the in vitro biomechanical performance of new injectable nano-hydroxyapatite （n-HA）/ polyamide 66 composite cements different in n-HA content in the augmentation of osteoporotic cancellous bone to choose suitable bone cement for the clinical applicability. METHODS： This experiment was performed in Chongqing University of Medical Sciences, Research Center For Nano-Biomaterials of Sichuan University and State Key Laboratory of Biotherapy of Sichuan University from January 2001 to January 2003. The cadaveric T1-T6 thoracic vertebrae and bilateral femur samples of five elderly people and one young people were obtained and were showed no pathologic lesion and damage by roentgenography. In axial compressive testing, the 34 vertebra/ bodies resected the disc and posterior parts were used.①The 28 elderly vertebral bodies were randomly divided into four groups, 60% n-HA/PA66 composite cement （HP1 group）, 70% n-HA/PA66 composite cement （HP2 group）, 80% n-HA/PA66 composite cement （HP3 group） and osteoporosis group, respectively with 7 specimens in each group. The 6 young vertebral bodies were used as normal control group. ②Vertebroplasty was performed with 5 mL 60%, 70% and 80% n-HA/PA66 composite cements in HP1, HP2 and HP3 groups, respectively. Those in the osteoporosis and normal control groups were only punctured. ③The loading-displacement and pressure-displacement data of every sample were measured, and sampling frequency was 10 Hz. In the torsional testing, the cancellous bone of 10 femoral condyles was used. ①Randomly, 8 elderly femoral condyles were divided into 4 groups： HP1, HP2, HP3 groups and osteoporosis group, 2 condyles in every group; the 2 young femoral condyles were regarded as normal control group. ②Those in the HP1 ,HP2 and HP3 groups were injected compressively with 20 mL 60%, 70% and 80% n-HA/PA66 cement, respectively. Those in the osteoporosis and normal control groups were only punctured. The cancellous bones of condyles were cut into about 10 mm×1

关 键 词：骨质疏松 羟基磷灰石类 酰胺类 注射 骨粘合剂 治疗应用 聚酰胺66 骨水泥 松质骨性能 生物力学 

分 类 号：R681[医药卫生—骨科学]