大鼠小肠移植排斥反应时外周血T淋巴细胞上CD2分子的表达  

The role of CD2 in acute rejection after small bowel transplantation in rats

作　　者：季刚[1] 林艳[1] 王为忠[1] 管文贤[1] 牛卫博[1] 

机构地区：[1]第四军医大学附属西京医院胃肠外科,西安710032

出　　处：《中华器官移植杂志》2006年第1期49-52,共4页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金资助(30070742)

摘　　要：目的探讨大鼠小肠移植急性排斥反应时外周血T淋巴细胞上CD2分子的表达。方法实验分3组进行,A组为假手术对照组(n=18),给予普通饲料喂养;B组(n=18)行SD大鼠到SD大鼠的同系小肠移植,术后常规补液,给予抗生素;C组(n=18)行SD大鼠到Wistar大鼠的小肠移植,术后处理同B组。各组于术后3、5、7d取肝素抗凝血,行流式细胞术检测,同时取移植肠组织,进行病理学检查。结果术后C组动物的存活时间为(7.0±2.1)d,B组为(33.3±2.3)d,A组>90d,C组与A、B组相比,差异有统计学意义(P<0.05);术后3、5、7d的外周血CD2阳性T淋巴细胞,A组分别为70.2%、69.8%和70.3%;B组为71.3%、69.7%和70.2%;C组为95.6%、88.1%和81.2%,C组各时点的CD2阳性细胞均高于A、B组相应时点(P<0.05);C组移植肠可见排斥反应的病理改变,且随术后时间的延长逐渐加重。结论小肠移植术后发生急性排斥反应时外周血CD2阳性T淋巴细胞表达率升高;术后早期CD2表达率的突然增高,提示可能发生急性排斥反应。Objective To investigate the expression of CD2 in peripheral blood T lymphocytes in acute rejection after small bowel transplantation （SBT） in rats. Methods Three groups were set up： Group A （n= 18）, receiving sham operation and feeding of common feedstuff; Group B （n = 18） receiving SD rat to SD rat iso-series SBT and the treatment of routine liquid infusion and antibiotics after operation; Group C （n = 18） receiving SD rat to Wistar rat taniso-series SBT and the same postoperation treatments as in group B. Samples of grafts and peripheral blood in the three groups were collected on the day 3, 5 and 7 respectively after SBT for detection of flow cytometry. The intestinal grafts were obtained for pathological examination. Results The survival time of rats in group C, group B and group A was 7. 0 ± 2. 1, 33.3 ± 2. 3 and more than 90 days, respectively. There was significant difference between group C and groups A, B （P〈0.05）. The percentage of CD2 positive T lymphoeytes in peripheral blood 3, 5 and 7 days after operation was 70. 2 %, 69.8 % and 70. 3 % in group A, 71.3 % ,69. 7 % and 70. 2 % in group B and 95.6 %, 88. 1% and 81.2 % in group C respectively. The percentage of CD2 positive T lymphocytes in group C was significantly higher than that in groups A and B （P〈0.05）. The pathological changes of the intestinal grafts in group C were observed and gradually deteriorated with the postoperative time over. Conclusion A sudden rise of the CD2 positive T lymphocytes intensively suggested the occurrence of acute rejection after SBT and could be thought of as one of immune markers which can predict the occurrence of acute rejection in the early stage.

关 键 词：小肠 移植 CD2抗原 大鼠 

分 类 号：R656.7[医药卫生—外科学]