整合素在增生性瘢痕挛缩过程中作用机制的实验研究  

The experimental study of mechanism of integrin in hypertrophic scar contracture

作　　者：何征宇[1] 侯晓宾[1] 范志宏[1] 

机构地区：[1]第二医科大学附属仁济医院整形外科,上海200127

出　　处：《中华整形外科杂志》2006年第1期38-40,共3页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金资助(基金编号:3000017)

摘　　要：目的了解整合素在瘢痕挛缩过程中的作用。方法取10例增生性瘢痕标本进行成纤维细胞原代培养,通过荧光定量PCR法(FQ-PCR)测定经整合素和黏着斑激酶(FAK)抗体阻断后培养的瘢痕成纤维细胞中-αSMA mRNA表达量的变化。结果经整合素和FAK抗体阻断后培养的成纤维细胞-αSMA的基因拷贝数均较阴性对照组明显下降(P<0.05)。结论整合素及FAK通过促进-αSMA的合成引起瘢痕的挛缩,在瘢痕挛缩过程中具有重要的作用。Objective To stud） the mechanism of integrin in hypertrophic scar. Methods fibroblasts from 10 samples of human hypertrophic scars was cultured, FQ-PCR assay was applied to detect mRNA expression of α-SMA in hypertrophic scar fibroblasts after integrin and FAK antibody blocking. Results mRNA of α-SMA in fibroblasts expressed obviously lower after integrin and FAK antibody blocking than that of control groups （ P 〈 0.05） . Conclusion Through accelerating the synthesis of α-SMA, integrin and FAK play an important role in contracture of hypertrophic scar.

关 键 词：增生性瘢痕 整合素 Α-平滑肌肌动蛋白 

分 类 号：R622[医药卫生—整形外科]