骨髓基质干细胞转染腺病毒BMP7后的成骨改变  

Effect of adeno-BMP7 transfection on osteogenesis of BMSCs

作　　者：祝联[1] 陈付国[1] 刘伟[1] 崔磊[1] 周广东[1] 刘德莉[1] 曹谊林[1] 

机构地区：[1]上海交通大学附属第九人民医院整形外科上海市组织工程研究中心,200011

出　　处：《中华整形外科杂志》2006年第1期59-62,共4页Chinese Journal of Plastic Surgery

基　　金：国家"973"组织工程基本科学问题(G1999054304);国家"863"组织工程化骨构建技术与产品开发(2002AA20511)

摘　　要：目的 探讨BMP7腺病毒转染对骨髓基质干细胞（BMSCs）的体外、体内成骨功能的影响。方法 用重组骨形成蛋白7腺病毒（adenovirus bone morphogenetic protein7，Adeno-BMP7）转染（M．O．I=100）70％～80％融合时的第二代的羊骨髓基质干细胞，3d后分别进行免疫印迹（Westem—blot）、钙结节染色和珊瑚、细胞复合物皮下回植。结果 Western—blot检测表明转染Adeno—BMP7后的BMSCs有BMP7蛋白水平上的表达；钙结节染色表明转染Adeno—BMP7后的BMSCs可形成较大的钙结节。转染Adeno—BMP7的BMSCs可明显促进皮下新骨的形成。结论 Adeno—BMP7转染可促进BMSCs的体外成骨定向分化，Adeno—BMP7转染后的BMSCs具有更强的体内成骨能力。Objective To study the effect of adeno-BMP7 transfection on the biology of bone marrow stromal cells（BMSCs）. Methods Bone marrow was obtained from the goat. The BMSCs were isolated and cultured at the second passage. Once the cells attached and formed a monolayer with 70% - 80% confluency,adeno-BMP7 （M. O. I. = 100） was added to the cells. After three days, calcium node was examined with staining; cell-coral compound was replanted subcutaneously. Results With adeno-BMP7 transfection, BMP7 expression was detected with Western-blot; big calcium nodes were observed with staining. New bone formation was enhanced, which was evaluated by X-ray and histological examinations. Conclusions BMSCs transfected with adeno-BMP7 show much stronger osteogenic ability.

关 键 词：重组腺病毒 骨形成蛋白7 骨髓基质干细胞 组织工程 

分 类 号：R318.0[医药卫生—生物医学工程]