齿兰环斑病毒的鉴定及其抗血清的制备与应用  

Identification and Serological Study of Odontoglossum ringspot virus

作　　者：明艳林[1] 郑国华[1] 李梅[1] 

机构地区：[1]厦门华侨亚热带植物引种园国家农作物国外引种隔离检疫基地,福建厦门361002

出　　处：《中国病毒学》2006年第1期64-67,共4页Virologica Sinica

基　　金：福建省自然科学基金资助项目(B9910027);厦门市科技创新资金计划项目(3502Z20031037)

摘　　要：根据病毒生物学特性、粒体形态和血清学性质等初步确定从在厦门地区种植的表现为褪绿、褐色坏死斑症状的卡特兰(Cattleya)病株上分离的一个病毒分离物为齿兰环斑病毒(Odontoglossumringspotvirus,ORSV)。通过摩擦接种6科27种(或品种)指示植物,结果其中4科8种(或品种)表现症状,与烟草花叶病毒不同;理化性质试验表明稀释限点为5×10-5,致死温度为92℃～94℃,体外存活期超过3个月。提纯病毒为长约300nm,宽约18nm杆状粒体。SDS-PAGE电泳分析表明其外壳蛋白分子量约为17kDa。该分离物与ORSV抗血清具有强阳性反应、而与TMV抗血清反应微弱,认为该病毒为ORSV分离物。用纯化病毒免疫家兔,成功地制备出特异性抗血清,并通过Dot-ELISA法对厦门地区种植的兰花病株进行了检测研究。A virus isolated from cattleya in Xiamen, China was identified as an isolate of Odontoglossum ringspot virus, based on its biological properties, particle morphology, serological characterization and RT-PCR. Mechanical inoculation test with the isolate was carried out to 27 species of 6 families of indicative plants, of which 8 species of 4 families showed symptoms. The result is different from that of Tobacco mosaic virus. The thermal inactivation point of the isolate was about 92℃-94℃, and the longevity in vitro was beyond 3 months. Electron microscopic examination of the purified virus particle preparations showed straight particles of about 300nm long and 18nm wide. The pure viral coat protein was estimated to be about 17 kDa. The serological test showed that the virus isolation reacted positively with the antiserum against ORSV while weakly with TMV antiserum. Antiserum was prepared by immunizing rabbit, and used to detect ORSV in Xiamen orchid＇s plant by Dot-ELISA.

关 键 词：卡特兰 鉴定 抗血清 齿兰环斑病毒 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]