鸡传染性支气管炎病毒CK/CH/LDL97Ⅰ/97株S基因特征  

Characterization of Spike Gene of Infectious Bronchitis Coronavirus CK/CH/LDL97Ⅰ/97 Isolated in China

作　　者：刘昕[1] 刘胜旺[1] 孔宪刚[1] 张庆霞[1] 荣骏弓[1] 韩宗玺[1] 邵昱昊[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室

出　　处：《中国病毒学》2006年第1期68-70,共3页Virologica Sinica

基　　金：黑龙江省政府博士后科研启动金资助项目(LRB-KY01045)

摘　　要：The spike gene(S)of Infectious bronchitis virus(IBV)CK/CH/LDL97Ⅰ/97 isolate was amplified,cloned,sequenced and analyzed.Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids.The precursor of the S protein was cleaved into S1 and S2 fragments,which comprised 541 and 626 amino acid residues,respectively.The cleavage site sequence was RRTGR.The homologies of nucleotide and amino acid sequences of S1 gene between CK/CH/LDL97 Ⅰ/97 and 27 reference IBV strains ranged from 60.6% to 99.6%,and 50.5% to 99.1%,respectively.The CK/CH/LDL97Ⅰ/97 S1 was most similar to that of three proventriculus IBV isolates,Q1(99.1%),J2(98.9%),T3(98.9%),which were isolated in China.Phylogenetic analysis of S1 proteins indicated that CK/CH/LDL97Ⅰ/97,Q1,J2 and T3 formed a separated cluster,which might belong to a novel genotype of IBV.The spike gene （S） of Infectious bronchitis virus （IBV） CK/CH/LDL97 Ⅰ/97 isolate was amplified, cloned, sequenced and analyzed. Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids. The precursor of the S protein was cleaved into S1 and S2 fragments, which comprised 541 and 626 amino acid residues, respectively. The cleavage site sequence was RRTGR. The homologies of nucleotide and amino acid sequences of S1 gene between CK/CH/LDL97 Ⅰ/97 and 27 reference IBV strains ranged from 60.6% to 99.6%, and 50.5% to 99.1%, respectively. The CK/CH/LDL97 Ⅰ/97 S1 was most similar to that of three proventriculus IBV isolates, Q1（99.1%）, J2（98.9%）, T3 （98.9%）, which were isolated in China. Phylogenetic analysis of S1 proteins indicated that CK/CH/LDL97 I/97, Q1, J2 and T3 formed a separated cluster, which might belong to a novel genotype of IBV.

关 键 词：传染性支气管炎病毒 S糖蛋白 基因特征 

分 类 号：S852.65[农业科学—基础兽医学]