三磷酸腺苷对国人食管癌Eca-109和肝癌SMMC-7721细胞增殖的影响  

Effects of adenosine triphosphate and adenosine on the proliferation of Chinese esophageal carcinoma Eca-109 and hepatoma SMMC-7721 cells

作　　者：秦葵[1] 任雷鸣[1] 

机构地区：[1]河北医科大学药学院药理学研究室,河北石家庄050017

出　　处：《中国药理学通报》2006年第1期31-34,共4页Chinese Pharmacological Bulletin

基　　金：国家人事部留学回国人员科技活动择优资助项目(No2002-2)

摘　　要：目的 研究三磷酸腺苷（ATP）对人食管癌细胞株Eca-109和人肝癌细胞株SMMC-772l细胞增殖的影响。方法 采用MTT法测定ATP、腺苷（ADO）和三磷酸尿苷（UTP）抑制肿瘤细胞增殖的作用，Wrights-Giemsa染色观察细胞形态学的改变。结果ATP（0.03～0．3mmol·L^-1）和ADO（0．1～0.3mmol·L^-1）可不同程度地抑制Eca-109和SMMC-7721的增殖，ATP对两株肿瘤细胞增殖的抑制作用均强于ADO。ATP和ADO对Eca-109细胞的最大抑制率分别为86．36％和29.88％，IC50分别为0．056和0.823mmol·L^-1；对SMMC-7721细胞的最大抑制率分别为82．06％和52．84％，IC50分别为0．218和0．517mmol·L^-1。UTP对Eta-109细胞有很弱的抑制增殖作用，最大抑制率仅为18．27％；对SMMC-7721无抗增殖作用．两株细胞经高浓度（0．3mmol·L^-1）ATP处理后，部分SMMC-7721细胞形态出现了明显的凋亡特征，而Eta-109细胞凋亡特征不明显。结论ATP具有较强的抗Eca-109细胞增殖作用，其抗增殖作用主要由ATP自身所致，代谢产物ADO也具有一定的作用；而对于SMMC-772l细胞，ATP可能较大程度地通过降解为ADO而发挥抗增殖作用．Aim To study the effects of adenosine triphosphate （ATP） on the proliferation of Chinese esophageal carcinoma Eca-109 and hepatoma SMMC- 7721 cells. Methods MTF assay was used to determine the inhibition of the proliferation of the two cultured tumor cell lines in vitro by ATP, adenosine （ADO） and ufidine triphosphate （UTP）. Morphological changes of the two cell lines induced by ATP were observed under light microscope. Results ATP （0.03～0.3 mmol·L^-1） and ADO （0.1 ～0.3 mmol · L^-1 ） had inhibitory effects on Eca-109 and SMMC- 7721 cells concentration-dependently, and the inhibition by ATP was stronger than that by ADO in both cell lines. For Eca-109 cell line, the maximal inhibition rate of the proliferation by ATP and ADO was 86.36% and 29.88%, and the IC50 was 0.056 and 0.823 mmol · L^-1, respectively. For SMMC-7721 cell line,the maximal inhibition rate of the proliferation by ATP and ADO was 82.06% and 52.84%, and the IC50 was 0.218 and 0.517 mmol · L^-1, respectively. UTP had a very weak inhibitory effect on Eca-109 cell line, with the maximal inhibition rate of 18.27%, and did not significantly affect SMMC-7721 cell line. Exposed to higher concentration （0.3 mmol · L^-1 ） of ATP for 72 h, SMMC-7721 cells displayed morphological changes of apoptosis, but Eca-109 cells did not show the characteristics of apoptosis markedly. Conclusion ATP has a strong inhibitory effect on the proliferation of Eca-109 cell line, which is mainly induced by ATP per se, and a metabolite ADO also has weaker effects. For SMMC-7721 cell line, however, ATP inhibits the cell proliferation mainly via its degradation to ADO.

关 键 词：三磷酸腺苷 腺苷 三磷酸尿苷 抗肿瘤作用 细胞凋亡 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]