糖肾灵及贝那普利对糖尿病大鼠肾脏病变的影响及机制研究  

Effects of Tangshenling Mixture and benazepril on rats with diabetic nephropathy and its mechanism

作　　者：贺学林[1] 李剑平[2] 陈以平[3] 张志刚[4] 林维勤[1] 陈江华[1] 

机构地区：[1]浙江大学医学院第一医院肾脏病中心,浙江杭州310003 [2]浙江大学医学院第一医院中医科,浙江杭州310003 [3]上海中医药大学龙华医院肾内科,上海200032 [4]复旦大学上海医学院病理学教研室,上海200032

出　　处：《中西医结合学报》2006年第1期43-47,共5页Journal of Chinese Integrative Medicine

基　　金：浙江省中医药管理局资助项目(No.2002C074);浙江省中医药管理局重点学科资助项目(No.A-402)

摘　　要：目的:研究糖肾灵合剂(Tang shen ling Mixture,TSLM)及贝那普利(benazepril)对糖尿病(diabetes mellitus,DM)大鼠肾脏病变的影响及其作用机制。方法:采用链脲霉素(strep to zotocin,STZ)诱导DM大鼠肾脏病变模型,进行6周的药物干预,观察大鼠血和尿的生化指标、血浆心钠素(atrial natriuretic factor,ANF)、肾脏病理变化、肾组织转化生长因子β1(transforming growth factorbeta1,TGF-β1)及葡萄糖转运因子1(glucose transporter1,GLUT1)mRNA的表达。结果:TSLM和贝那普利均能降低DM大鼠尿白蛋白排泄率、肌酐清除率及肾重/体重比,减轻肾脏病理损害。TSLM能降低血浆ANF水平和肾组织GLUT1mRNA的表达,对肾组织TGF-β1mRNA表达的影响则不显著。贝那普利能降低肾组织TGF-β1mRNA的表达,对血浆ANF和肾组织GLUT1mRNA表达的影响则不显著。结论:TSLM能改善DM大鼠早期肾脏病变,其作用机制与贝那普利不同,可能与降低血浆ANF和肾组织GLUT1mRNA的表达等有关。TSLM与贝那普利合用具有一定的协同作用。Objective： To investigate the effects of Tangshenling Mixture （TSLM） and benazepril on rats with diabetic nephropathy （DN） and its mechanism. Methods： Diabetic nephropathy was induced in rats by intraperitoneal injection of streptozotocin. Fifty-eight rats with DN were randomly divided into four groups： untreated group, TSLM-treated group, TSLM plus benazepril-treated group and benazepril-treated group. Another seven normal rats were included in normal control group. Then, rats in each group were accordingly given normal saline, TSLM, TSLM plus benazepril and benazepril orally for six weeks respectively. Blood and urine biochemical indexes, plasma atrial natriuretic factor （ANF）, pathomorphology of renal tissue, transforming growth factor beta1 （TGF-β1） and glucose transporter 1 （GLUT1） mRNAs in renal tissue were observed. Results： Both TSLM and benazepril could decrease urinary albumin excretion rates, creatinine clearance and ratio of kidney weight to body weight of the rats with DN as well as reduce the pathological damages of the renal tissues. TSLM could reduce the level of plasma ANF and the expression of GLUT1 mRNA, but had no significant effect on the expression of TGF-β1 mRNA. Benazepril could reduce the expression of TGF-β1 mRNA, but had no significant effect on plasma ANF and the expression of GLUUT1 mRNA. Conclusion： TSLM can reduce the pathological damages of renal tissues in rats with early-stage DN, and its mechanism may relate to decreasing the level of plasma ANF and the expression of GLUT1 mRNA which is different from that of benazepril. It seems that TSLM has synergetic effect with benazepril.

关 键 词：链脲霉素糖尿病 糖肾灵合剂 贝那普利 转化生长因子Β1 葡萄糖转运因子1 

分 类 号：R587.2[医药卫生—内分泌]