肝再生中α2-巨球蛋白的表达动态分析  

Expression Change of Alpha-2-macroglobulin in Liver Regeneration

作　　者：袁金云[1] 耿飒[1] 姬生栋[1] 徐存拴 

机构地区：[1]河南师范大学生命科学院

出　　处：《医学分子生物学杂志》2006年第1期13-16,21,共5页Journal of Medical Molecular Biology

基　　金：国家自然科学基金(No.30270673)~~

摘　　要：目的确定更多的与肝再生相关的基因，方法借助4～8正向抑制性消减文库（0-4-8-12短间隔连续部分肝切除）产生的α2-巨球蛋白基因片段．运用cDNA芯片分析了其mRNA在再生肝中的表达动态。结果肝再生中α2-巨球蛋白呈上调表达．而且变化幅度很大，至高点超过21倍。大部分肝切除中α2-巨球蛋白的表达高峰出现在8h和16h.短间隔连续部分肝切除中α2-巨球蛋白的表达呈现不同的变化趋势．4h短间隔的连续肝切除诱导α2-巨球蛋白表达不断升高．而第二次的36h短间隔的连续肝切除才能诱导α2-巨球蛋白表达显著升高。结论肝再生中α2-巨球蛋白可能在早期的正相急性反应中起重要作用，减少切除伤害带来的炎症．促进肝再生的顺利进行。Objective To identify more genes related to liver regeneration bv using eDNA miero assay （ rat liver eDNA chip）. Methods This study analyzed expression change of alpha-2-maeroglobulin mRNA from 4-8 forward （0-4-8-12 h short interval successive partial hepatectomy） subtractive eDNA libraries in regenerating rat liver. Results Alpha-2-maeroglobulin was largely up-regulated in liver regeneration and was more than 21 times normal level at the expression peak. Expression peaks of alpha-2-macroglobulin appeared 8 h and 16 h after partial hepatectomy （PH）. Expression change of alpha-2-maeroglobulin during SISPH presentsed different profiles in contrast to that of PH. Successive hepatectomy with 4 h interval could induce successive increase of alpha-2-macroglobulin and only the second successive hepatectomy after 36 h could induce marked increase. Conclusion The alpha-2-maeroglobulin might play an important role in early stress response in alleviating inflammation induced by hepatectomy and ensuring liver regeneration.

关 键 词：肝再生 Α2-巨球蛋白 肝切除 抑制性消减杂交技术 CDNA芯片 

分 类 号：Q51[生物学—生物化学]