猪1型圆环病毒全基因组克隆及其感染性初步鉴定  

Genome Cloning and Infectivity Identification of Porcine Circovirus Type 1

作　　者：曹胜波[1] 刘学芹[1] 刘朝[1] 孙敏轩[1] 陈焕春[1] 

机构地区：[1]华中农业大学动物医学院动物病毒室,湖北武汉430070

出　　处：《病毒学报》2006年第1期66-69,共4页Chinese Journal of Virology

基　　金：国家自然科学基金资助(36170701)

摘　　要：PCV1 was isolated from IBRS-2 cells line,and its complete genomic sequence was cloned by PCR.Sequence analysis indicated that this PCV1 strain shares >98% nucleotide identity with the other PCV1 strains in GenBank.Then double copy molecule clone(pSK2PCV1) was constructed and used to transfect PK-15 cell line.The results of indirect immunofluorescence(IIF) and RT-PCR suggested that pSK2PCV1 could form infectious virus after being transfected into PK-15 cells.PCV1 was isolated from IBRS-2 cells line, and its complete genomic sequence was cloned by PCR. Sequence analysis indicated that this PCV1 strain shares 〉98 % nucleotide identity with the other PCV1 strains in GenBank. Then double copy molecule clone （pSK2PCV1） was constructed and used to transfect PK-15 cell line. The results of indirect immunofluorescence （IIF） and RT-PCR suggested that pSK2PCV1 could form infectious virus after being transfected into PK-15 cells.

关 键 词：猪1型圆环病毒(PCV1) 全基因组 感染性 

分 类 号：S850[农业科学—兽医学]