皇帝蕉薄片外植体愈伤组织的诱导及植株再生  

Induction of callus and organogenesis of Huangdi banana cultivar from the thin-cell-layer

作　　者：朱靖杰[1] 王宇光[2] 雷禄旺[2] 畅文军[2] 

机构地区：[1]华南热带农业大学园艺学院,海南儋州571737 [2]中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室,海口571101

出　　处：《果树学报》2006年第1期111-114,F0002,共5页Journal of Fruit Science

摘　　要：将皇帝蕉试管苗茎段徒手切成厚约1mm的薄片,经无菌的0.5%柠檬酸溶液处理片刻后,接入各种培养基中。结果表明:(1)适度的暗培养预处理有利于愈伤组织诱导,合适暗培养天数为4d;(2)诱导愈伤组织最佳培养基为:B5+2,4-D13.572μmol/L+IBA4.921μmol/L+NAA5.371μmol/L+KT13.94μmol/L+椰乳5%+PP3330.0001mg/L,愈伤组织诱导率为86.0%;(3)最佳愈伤组织分化芽培养基为:改良MS培养基+BA13.6831μmol/L+NAA0.537μmol/L,芽分化率达到87.0%;(4)诱导芽生根的最佳培养基是:1/2MS+IBA0.492μmol/L(或+NAA0.537μmol/L),生根率达到100%。After cut to 1 mm thin layer and immersed in 0.5% sterile citric acid for few minutes, the stems of the tube seedling of Huangdi banana（ Musa paradisica AA） in vitro were cultured in different media for induction of callus and organogenesis. The results indicated： （1） Moderate dark treatment was beneficial to induce the callus and the optimal treatment time was 4 days;（2） The optimal medium for callus induction was composed of B5 ＋ 2,4-D 13.570 μ mol/L＋ IBA 4.920 μ mol/L＋ NAA 5.371 μ mol/L ＋ KT13.94 μ mol/L ＋ 5% coconut milk ＋ PP333 0.0001 mg/L and the callus induction rate was 86.0%; （3） The optimal medium for shoot bud induction from the callus was composed of MS ＋ BA13.683 μ mol/L＋ NAA0.537 μ mol/L, and the rate of shoot polarization was 87.0%; （4）The optimal medium for root formation was composed of 1/2 MS ＋ IBA 0.492 μ mol/L （or 1/2 MS ＋ NAA 0.537 μ mol/L）, and the root formation rate was 100%.

关 键 词：皇帝蕉 横切薄层 愈伤组织 再生植株 

分 类 号：S668.1[农业科学—果树学]