Müller细胞对视网膜血管内皮细胞紧密连接蛋白表达的影响  

Effects of Müller cells on expression of occludin in retinal vascular endothelial cells

作　　者：叶辉 徐格致[1] 王文吉[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院,上海200031

出　　处：《中华眼底病杂志》2006年第1期28-30,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的探讨体外培养的Müller细胞在糖基化终末产物(AGEs)作用下增殖活性的变化,以及这种改变对牛视网膜血管内皮细胞(BREC)紧密连接蛋白(occludin)表达的影响。方法培养新生大鼠Müller细胞和BREC,两类细胞均用特异性抗体进行免疫鉴定。将培养的BREC分4组观察:第1组未添加任何细胞上清液;第2组添加正常Müller细胞上清液;第3组添加糖基化终末产物(AGEs)作用后的Müller细胞上清液;第4组无细胞组,为空白对照组。酶联免疫吸附法(ELISA)测定4组细胞上清液中紧密连接蛋白的含量,比较其变化。结果添加正常Müller细胞上清液组紧密连接蛋白表达量最多,未添加任何细胞上清液组次之,添加AGEs作用后的Müller细胞上清液组更少。结论AGEs能促进Müller细胞异常增殖、抑制BREC表达紧密连接蛋白。Obiective To investigate the change of the activity of proliferation in cultivated Mueller cells treated by advanced glycation endoproducts （AGEs）, and the effect of these changes on expression of occludin in bovine retinal vascular endothelial cells （BREC）. Methods The cultivated Mueller cells were devided into normal growth group and cultured with AGEs group. The cultured BREC were devided into 4 groups：group 1, without any medium; group 2, with normal growth Mueller cell medium （MCM）; group 3,MCM treated by AGEs; group 4, without cell as the control. Enzyme-linkedimmunosorbent assay was used to detect the content of occludin in the medium in the 4 groups. Results The content of expression of occludin was the most in group 2, less in group 1, and the least in group 3. Conclusion AGEs may promote the abnormal proliferation of Mueller cells and inhibit the expression of occludin in BREC.

关 键 词：糖基化终产物 高级 MUELLER细胞 内皮 血管 血视网膜屏障 

分 类 号：R587.2[医药卫生—内分泌]