寻常性银屑病患者外周血中性粒细胞中趋化因子受体的表达  

The expression of chemokine receptors on neutrophils in patients with psoriasis vulgaris

作　　者：杨桂兰[1] 陈志强[2] 郑家润[2] 李新宇[2] 陈沄[2] 李遇梅[2] 高建明[2] 王晓丹[2] 冯华[2] 

机构地区：[1]中国人民解放军兰州军区兰州总医院皮肤科,甘肃兰州733050 [2]中国医学科学院,中国协和医科大学皮肤病研究所,江苏南京210042

出　　处：《临床皮肤科杂志》2006年第2期71-73,共3页Journal of Clinical Dermatology

基　　金：卫生部科技专项基金资助项目(981041)

摘　　要：目的:了解趋化因子受体在寻常性银屑病外周血中性粒细胞活化及趋化中的作用,探讨寻常性银屑病的发病机制。方法:应用反转录(RT)-PCR检测33例进行期斑块状银屑病患者及其中16例患者治疗后外周血中性粒细胞中趋化因子受体CXCR1～3及CCR1、5mRNA的表达水平,并设30名正常人作对照。将检测结果与银屑病皮损面积严重度指数(PASI)进行相关性分析。结果:寻常性银屑病患者治疗前中性粒细胞中CXCR1、CXCR2与CCR1mRNA表达水平分别为1.36±1.19、1.45±0.87与1.43±1.28,明显高于正常对照及治疗后的患者[正常对照分别为0.55±0.37(P<0.01),0.74±0.58(P<0.001),0.68±0.37(P<0.001);治疗后分别为0.46±0.33(P<0.001),0.63±0.58(P<0.01),0.73±0.86(P<0.05)],其中CXCR1、CXCR2与PASI呈显著正相关。结论:CXCR1、CXCR2与CCR1在寻常性银屑病患者外周血中性粒细胞中表达异常增高,它们可能参与了中性粒细胞的活化及趋化,进而参与了银屑病的发病。Objective： To investigate the expression of ehemokine receptors on neutrophils in plaque type psoriasis and in progressive stage its clinical significance. Methods： Reverse transeription-polymerase chain reaetion（RT-PCR） technique was applied to semi-quantitatively analyze CXC type ehemokine receptors CXCR1-3 and CC type ehemokine receptors CCR1 and CCR5 mRNA expression on peripheral blood neutrophils in 33 eases of psoriatic patients, 16 of the 33 patients who were treated until 70% of the skin lesions resolved, and 30 healthy controls. The correlations between CXCR1, CXCR2 and CCR1 mRNA expression and psoriatic area and severity index （PASI） were evaluated, respectively. Results： The expression levels of CXCR1, CXCR2 and CCR1 mRNA on neutrophils from psoriatic patients were 1.36±1.19, 1.45±0.87 and 1.43±1.28, respectively. which were significantly higher than those in normal controls [0.55±0.37 （P〈 0.01）, 0.74±0.58 and 0.68±0.37 （ both P〈 0.001）, respectively] and post-treatment patients[0.46±0.33（P〈0.001）, 0.63±0.58（P〈0.01） and 0.73±0.86（P〈0.05）, respectively]. A positive correlation was observed between both CXCR1 and PASI, and CXCR2 and PASI. Conclusions： The increased ex- pression of CXCR1/2 and CCR1 maybe associated with migration and aggregation of neutrophils in psoriatic lesional epidermis, suggesting that these three ehemokine receptors be involved in the pathogenesis of plaque type psoriasis in progressive stage.

关 键 词：银屑病 中性粒细胞 受体 趋化因子 

分 类 号：R758.63[医药卫生—皮肤病学与性病学] R446.62[医药卫生—临床医学]