东海微生物中6种环二肽类天然活性物质的分离和鉴定  

Bioactive cyclodipeptides extracted from marine microbes in East China Sea

作　　者：艾峰[1] 许强芝[1] 杨妤[1] 刘小宇[1] 施晓琼[1] 宋志刚[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础医学部生物化学与分子生物学教研室,上海200433

出　　处：《第二军医大学学报》2006年第1期22-24,共3页Academic Journal of Second Military Medical University

基　　金：上海市优秀学科带头人计划(05XD1423).~~

摘　　要：目的:从海洋微生物中寻找天然活性物质。方法:利用稻瘟霉作为菌株筛选模型,对筛选出来的活性菌株F8712发酵液的醋酸乙酯提取物进行化合物分离;通过1HNMR1、3CNMR和ESI-MS对化合物进行结构鉴定。结果和讨论:鉴定了6个环二肽类化合物,它们分别为环[Ala-Leu](Ⅰ),环[Ala-Ile](Ⅱ),环[Val-Pro](Ⅲ),环[Leu-Pro](Ⅳ),环[Ile-Pro](Ⅴ)和环[Leu-Val](Ⅵ)。这些环二肽都没有抗稻瘟霉活性,化合物Ⅴ对鳗弧菌(Vibrio anguillarm)具有较强的抑制能力,其MIC为0.07μg/ml。Objective：To search for bioactive compounds from marine microbes. Methods： Pyricularia oryzae was used to screen microbes. Compounds extracted with EtOAc from the fermentation broth of F8712 strain（an active microbe screened out） were separated. The chemical structures of the separated compounds were identified by ^1HNMR, ^13CNMR and ESI-MS tech niques. Results：Six cyclic dipeptides, cyclo（Ala-Leu）, cyclo（Ala-Ⅱe）, cyclo（Ⅱe-Pro）, cyclo（Val- Pro）, cyclo（Leu-Pro） and cyclo（Leu- Val）, were obtained. Conclusion： None of the 6 cyclodipeptides has any anti-Pyricularia oryzae activities. Compound V has potent inhibitory effects on Vibrio allguillarm with a minimum inhibitory concentration of 0.07 μg/ml.

关 键 词：环二肽 海洋生物学 稻瘟霉 鳗弧苗 分离 鉴定 东海 

分 类 号：R282.77[医药卫生—中药学]