海螵蛸多糖CPS-1对小鼠实验性溃疡性结肠炎作用的初步观察  

Effects of Cuttlebone polysaccharide CPS-1 on experimental ulcerative colitis in mice

作　　者：魏江洲[1] 张建鹏[1] 刘军华[1] 徐红丽[1] 冯伟华[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础医学部生物化学与分子生物学教研室,上海200433

出　　处：《第二军医大学学报》2006年第1期28-30,共3页Academic Journal of Second Military Medical University

基　　金：国家重点科技攻关计划(96-C-02-01-12);浙江省科技计划项目(2005ZJKJJH2202).~~

摘　　要：目的:探讨海螵蛸多糖相对均一组分———CPS-1对小鼠实验性溃疡性结肠炎(UC)的保护作用。方法:采用DSS诱导法建立小鼠UC模型,以SMZ-TMP为对照,通过临床症状、体质量改变、局部肠黏膜(溃疡发生)改变情况等指标对高、低剂量CPS-1对UC小鼠的保护作用进行初步观察。并采用ELISA方法测定了小鼠血清中某些内皮增殖及炎症相关的细胞因子的含量变化。结果:海螵蛸多糖治疗组小鼠模型建立后的体质量没有明显降低,小鼠的一般生长情况都良好,血清中EGF和PDGF的含量较模型建立前明显增加,而TNF-α的含量与阳性对照组相比则明显降低(P<0.05)。结论:海螵蛸多糖CPS-1能够明显的提高UC小鼠血液中EGF和PDGF的含量,加速溃疡组织的愈合;同时降低TNF-α的表达,从而缓解炎症。因此,海螵蛸多糖CPS-1能够加速溃疡组织的愈合和修复过程。Objective：To study the protective effects of cuttlebone polysaccharide CPS-1 on experimental ulcerative colitis （UC） in mice. Methods： Dextran sodium sulfate （DSS） was used to induce UC in mice and mice treated with SMP＋TMP were taken as control. The protective effects of cuttlebone polysaccharide CPS-1 （high and low dose） on experimental UC in mice were evaluated with clinical symptoms, body weight changes and pathological changes in local intestinal mucous membrane. Cytokines related to endothelial cell proliferation and inflammation were determined in mice serum using ELISA kits. Results： There was no obvious change in body weight in mice treated with CPS-1 and the mice were well alive. Serum EGF and PDGF in CPS-1 treated mice were obviously increased compared with those in normal mice, while serum TNF-α in CPS-1 treated mice was significantly lower than that of positive control mice（P〈0.05）. Conclusion： Cuttlebone polysaccharide CPS-1 can obviously increase EGF and PDGF levels in experimental UC mice and accelerate the concrescence and repair of ulcer. Meanwhile,it can also decrease the expression of TNF-α and relieve inflammation.

关 键 词：海螺蛸 多糖类 结肠炎 溃疡性 小鼠 

分 类 号：R282.77[医药卫生—中药学] R574.62[医药卫生—中医学]