钙拮抗剂减轻大鼠皮瓣缺血再灌注损伤的效应  

Effect of calcium antagonist in relieving ischemia reperfusion injury in skin flap of rats

作　　者：宋赫[1] 高丽萍[1] 李志海[1] 杨国华[1] 

机构地区：[1]哈尔滨医科大学第一临床医院急诊科,黑龙江省哈尔滨市150001

出　　处：《中国临床康复》2006年第4期78-79,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:对照应用与不应用钙离子拮抗剂大鼠皮瓣缺血再灌注损伤后某些特异性化学物质的含量,探讨钙离子拮抗剂对皮瓣缺血再灌注损伤的保护作用。方法:实验于2003/2004在哈尔滨医科大学动物实验室进行。实验大鼠33只,随机分成3组,每组11只。对照组:术前3d腹腔注射生理盐水,2mL/d,共用3d。维拉帕米组:术前3d腹腔注射维拉帕米16mg/(kg·d),共用3d。汉防已甲素组:术前3d腹腔注射汉防已甲素40mg/(kg·d),共用3d。在缺血前和缺血5h和再灌注20min后,检测皮瓣脂肪组织中超氧化物歧化酶活性、谷胱甘肽过氧化酶活性和丙二醛含量的变化。结果:实验大鼠33只均进入结果分析。①大鼠腹壁皮瓣超氧化物歧化酶活性:对照组再灌注20min低于缺血前[(228.92±22.4),(312.76±47.77)mmol/L,P<0.01],维拉帕米组和汉防己甲素组缺血5h低于对照组[(293.30±28.5),(290.47±29.3),(265.74±29.3)mmol/L,P<0.05],维拉帕米组和汉防己甲素组再灌注20min高于对照组[(332.94±41.5),(334.89±41.6)mmol/L,P<0.01]。②大鼠皮瓣脂肪组织中的谷胱甘肽过氧化酶活性:对照组皮瓣在缺血5h,再灌注20min的谷胱甘肽过氧化酶活性,比缺血前明显降低(P<0.05或0.01)。再灌注20min时,维拉帕米和汉防己甲素组的谷胱甘肽过氧化酶活性均较对照组明显升高。③大鼠皮瓣脂肪中的丙二醛含量:对照组皮瓣缺血5h时脂肪中丙二醛的含量与缺血前相比无明显差异(P>0.05),再灌注20min时,丙二醛含量比缺血前明显增高(P<0.01)。维拉帕米和汉防己甲素组再灌注20min时丙二醛含量比对照组明显下降(P<0.01)。结论:维拉帕米和汉防己甲素可以明显地提高皮瓣的超氧化物歧化酶活性和谷胱甘肽过氧化酶活性,并可显著降低丙二醛的含量。钙离子拮抗剂能减轻大鼠腹部皮瓣缺血再灌注损伤的脂质过氧化作用。应用钙离子拮抗剂在组织缺血再灌注损伤过程中可以抑制钙离子内�AIM： To compare the contents of some specific chemical substance after ischemia reperfusion injury in skin flap of rats treated with or without calcium antagonist, and investigate the protective effect of calcium antagonist on iscbemia reperfusion injury in skin flap of rats. METHODS： The experiment was carried out in the animal laboratory of Harbin Medical University between 2003 and 2004. Thirty-three experimental rats were randomized into three groups with 11 rats in each group. Control group： the rats were treated with intrapcritoneal injection of saline at 3 days preoperatively, 2 mL per day for 3 days, Verapamil group： the rats were given intraperitoneal injection of verapamil at 3 days preoperatively, 16 mg/kg per day for 3 days, Tetrandrine group： the rats were given intraperitoneal injection of tetrandrine at 3 days preoperatively, 40 mg/kg per day for 3 days, The changes of the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） and content of malondialdehyde （MDA） in the fatty tissue of skin flap were detected before ischemia and after ischemia for 5 hours and reperfusion for 20 minutes respectively. RESULTS： All the 33 rats were involved in the analysis of results. ①SOD activity in abdominal skin flap of rats： In the control group, it was obviously lower at 20 minutes after reperfusion than before ischemia [（228.92±22.4）, （312.76±47.77） retool/L, P 〈 0.01]. The SOD activity at 5 hours after ischemia was lower in the verapamil group and tetrandrine group than in the control group [（293.30±28.5）, （290.47±29.3）, （265.74±29.3）mmol/L, P 〈 0.05]. The SOD activity at 20 minutes after reperfusion was higher in the verapamil group and tetrandrine group than in the control group [（332.94±41.5）, （334.89±41.6）mmol/L, P 〈 0.01].②GSH-Px activity in abdominal skin flap of rats： In the control group, the GSH-Px activity at ischemia for 5 hours and reperfusion for 20 minutes was obviously decreased as compared with that

关 键 词：再灌注损伤 钙通道阻滞药 脂质过氧化作用 外科皮瓣 钙拮抗剂 皮瓣缺血 

分 类 号：R972[医药卫生—药品]