口服高氧液对急性光气中毒性肺损伤小鼠的干预效应  

Interventional effect of oral administration of hyperxia solution on phosgene-induced acute lung injury in mice

作　　者：王玲[1] 徐礼鲜[2] 唐时荣[1] 张惠[2] 王惠霞 

机构地区：[1]解放军第一五○医院麻醉科,河南省洛阳市471031 [2]解放军第四军医大学口腔医学院麻醉科,陕西省西安市710032 [3]解放军济南军区总院麻醉科,山东省济南市250031

出　　处：《中国临床康复》2006年第4期88-90,共3页Chinese Journal of Clinical Rehabilitation

基　　金：长江学者特配骨干教师计划;陕西省自然科学基金项目(99SM39)~~

摘　　要：目的:分析口服高氧液对双用途毒剂光气所致急性中毒性肺损伤小鼠的干预效应。方法:实验于2003-09/2004-04在第四军医大学预防医学系毒理学教研室完成。选择健康昆明种系清洁级小鼠60只,随机分为高浓度染毒组(n=30)、低浓度染毒组(n=20)和正常对照组(n=10)。制备动物染毒模型,固定染毒时间为3min。高浓度染毒组放入光气剂量为28.56mg/L染毒柜中;又分为平衡盐对照组(染毒后分别于1,3,5h经胃灌入平衡盐0.05mL/g)、高氧液治疗组(染毒后分别于1,3,5h经胃灌入高氧液0.05mL/g)和光气中毒组(染毒后不予干预),每组10只;低浓度染毒组放入光气剂量为14.88mg/L染毒柜中,分为平衡盐对照组和高氧液治疗组,两组均分别于染毒后1,3,5h经胃灌入平衡盐或高氧液0.05mL/g,每组10只;正常对照组放入相同染毒柜内吸入新鲜空气3min。高浓度染毒组记录动物染毒后生存时间,低浓度染毒组均在染毒后8h处死,处死前心脏取血,测定动脉血氧分压及血清肿瘤坏死因子α含量,开胸取肺,测定肺组织丙二醛、还原型谷胱甘肽含量及肺系数的变化,留取部分肺组织作组织病理学检查。结果:在实验过程中无动物死亡,全部进入结果分析。①高浓度染毒后,高氧液治疗组生存时间较光气中毒组、平衡盐对照组延长,但差异无显著性[(8.0±2.0),(6.45±1.99),(6.55±1.89)h]。②低浓度染毒组中平衡盐对照组动脉血氧分压进行性下降,肺系数、血清肿瘤坏死因子α含量升高,肺组织还原型谷胱甘肽含量下降、丙二醛含量增加,与正常对照组比较差异有显著性(P<0.05或P<0.01);高氧液治疗组肺系数明显低于平衡盐对照组(P<0.05);高氧液治疗组与平衡盐对照组相比,肺组织丙二醛、血清肿瘤坏死因子α含量显著降低(P<0.01),但还原型谷胱甘肽含量没有明显改变。③正常对照组肺泡大小均匀、结构完整,无充血、淤血和水肿,光气中毒及�AIM： To analyze the therapeutic effects of oral administration of hyperxia solution on acute lung injury induced by phosgene in mice. METHODS： The experiment was can-led out in the Department of Toxicology. School of Preventive Medicine. the Fourth Military Medical University of Chinese PLA from September 2003 to April 2004. Totally 60 healthy Kunming mice of clean degree were randomly divided into three groups： high concentration phosgene group （n=30）, low concentration phosgene group （n=20） and control group （n=10）. Animal models were established, and the fixed time for phosgene was 3 minutes. The high concentration phosgene group in which mice were exposed to 28.56 mg/L phosgene was subdivided into three groups with 10 mice in each： balanced salt control group （0.05 mL/g balanced salt was perfused via stomach at 1, 3 and 5 hours after phosgene respectively）, hyperxia solution treated group （0.05 mL/g hyperxia solution was perfused via stomach at 1, 3 and 5 hours after phosgene respectively） and phosgene group （no intervention）. The low concentration phosgene group in which mice were exposed to 14.88 mg/L phosgene was subdivided into two groups with 10 mice in each： balanced salt control group and hyperxia solution treated group, the mice were given 0.05 mL/g balanced salt or hyperxia solution perfused via stomach at 1.3 and 5 hours,after phosgene respectively. Mitre in the control group were put into the same phosgene chamber and breathed fresh air for 3 minutes. The survival time of each mouse was recorded in the high concentration phosgene group, and the mice in the low concentration phosgene group were killed at 8 hours after phosgene, blood sample was obtained from heart before being killed determination of arterial partial pressure of oxygen （PaO2） and serum content of tumor necrosis factor-alpha （TNF-α）. The lung was removed by opening chest to detect the contents of malondialdehyde （MDA） and reduced glutathione （GSH） in lung tissue and the change of p

关 键 词：光气 血气分析 自由基 谷胱甘肽 丙二醛 肿瘤坏死因子 口服高氧液 急性光气中毒性肺损伤 干预效应 

分 类 号：R563[医药卫生—呼吸系统]