生理状态下外周血白细胞穿孔素基因表达的荧光定量PCR检测  

Quantitative Real-time Detection of Spontaneous Perforin(PFR) Gene Expression in Peripheral Blood Mononuclear Cell(PBMC) by Polymerase Chain Reaction

作　　者：王茹[1] 陈佩杰[1] 董强刚[1] 

机构地区：[1]上海体育学院运动科学系,上海200438

出　　处：《中国运动医学杂志》2006年第1期57-59,共3页Chinese Journal of Sports Medicine

基　　金：国家科技攻关计划(2002BA904B04-6);上海市教委第四期重点学科和上海市第二期重点学科---运动人体科学学科建设项目(编号T0901);国家教育部高等学校优秀青年教师教学科研奖励计划共同资助

摘　　要：目的:建立穿孔素(perforin,PFR)基因表达的绝对定量实时PCR检测技术。方法:应用实时定量PCR方法检测25名健康运动员生理状态下处周血PFR mRNA的表达。主要结果和结论:应用实时定量PCR方法能够对健康受试者外周血白细胞自发性PFR mRNA表达进行定量分析,相关系数r=1。Objective To develop a method for quantitative real - time detection of perforin （PFR） using polymerase chain reaction （PCR）. Methods Gene expression of PFR in peripheral blood mononuclear cell （PBMC） from 25 healthy individuals was detected by PCR. An endogenous＂housekeeping＂ gene GAPDH was used to normalize the results. Results and Conclusion The spontaneous PFR mRNA expression in PBMC in a given sample could be measured by PCR with relativity correlation coefficient of r = 1.

关 键 词：穿孔素 信使核糖核酸 实时定量PCR 生理状态 外周血 白细胞 

分 类 号：R446.9[医药卫生—诊断学]