传代培养角膜细胞的染色体遗传变异率分析  

Chromosome Variation Analysis of Transformed Corneal Cell Lines

作　　者：马志敏[1] 金月娥[2] 任志华[2] 卢珞[2] 陆振虞[2] 

机构地区：[1]大理学院遗传学教研室,大理671000 [2]上海交通大学基础医学院医学遗传学教研室

出　　处：《上海交通大学学报（医学版）》2006年第1期76-77,共2页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的 分析传代培养角膜细胞染色体数目的变异率。方法 选择人角膜上皮细胞（HCE）、牛角膜内皮细胞（BCE）和兔角膜上皮细胞（RCE）细胞，采用直接法制备传代细胞染色体标本，常规Giemsa染色，1000倍光学显微镜下计数分裂相染色体数目，进行核型分析，统计染色体数目变异情况。结果 HCE染色体众数为56—65，为超二倍体；BCE染色体众数为27～34，为亚二倍体；RCE染色体众数为74～88，为超二倍体。与起源细胞相比，以上3种传代细胞染色体数目均发生了改变。结论 该项核型分析为HCE、BCE和RCE的功能研究提供了重要的参考信息。Objective To analyze the stability of chromosome variant ratio of three available transformed corneal cell lines. Methods Chromosome specimens of transformed cells including human corneal epithelial cells（HCE） , bovine corneal endothelial cells （BCE） and rabbit corneal epithelial cells （RCE） were prepared by a direct method using regular Giemsa staining. Chromosomes of cells in metaphase were counted under the microscope. Then, the variant ratio of chromosomes and their nuclear types were analyzed. Results The chromosome numbers were 56 to 65, 27 to 34 and 74 to 88 for HCE, BCE and RCE, respectively. Chromosome numbers in the three commonly used and transformed corneal cell lines were changed in comparison to their parent tissues. Conclusion Genotyping study may provide important information for using HCE,BCE,RCE in functional studies.

关 键 词：传代培养细胞 染色体变异 角膜细胞 

分 类 号：Q813.11[生物学—生物工程]