骨髓基质细胞-牙根-珊瑚羟基磷灰石复合体体内再植的初步观察  

Preliminary observation on the effects of implantation of BMSCs-root-CHA complex in dog

作　　者：涂小丽[1] 刘宏伟[2] 

机构地区：[1]广州南方医科大学珠江医院口腔科,510282 [2]上海同济大学口腔医学院

出　　处：《实用口腔医学杂志》2006年第1期45-47,共3页Journal of Practical Stomatology

基　　金：全军"十五"重点指令性课题[01L075](分题);广东省科技计划项目(C30704)

摘　　要：目的:评价体外构建的骨髓基质细胞(BMSCs)、珊瑚-羟磷灰石支架(CHA)、牙根复合体植入体内后牙根周组织的再生情况。方法:体外分离纯化的狗BMSCs分别与牙根及CHA支架共培养形成复合体后植入体内生长,16周后取材,组织切片观察牙根周组织的再生情况。结果:实验组较对照组所不同的是:有更趋成熟、致密的牙槽骨生成;牙根与牙槽骨之间形成了清晰的软组织间隙———类牙周膜结构,主要由纤维组织、毛细血管组成。结论:BMSC-CHA-牙根复合物植入体内后有效促进牙周组织的再生;组织工程学方法促进牙周组织再生具有可行性。Objective-To evaluate the periodontal tissue regeneration effect of implantation of bone marrow stromal cells （BMSCs）-root- coralline hydroxyapatite （CHA） complex. Methods： BMSCs of beagle dogs were in vitro cultured on CHA scaffold and on tooth root surface respectively. Then BMSCs-root samples were inserted into BMSCs- CHA scaffolds to form BMSCs-CHA-root complexes. 5 complexes were implanted on one side of mandible in each of the dogs 1 month after the 4th,5th and 6th teeth had been extracted. Root-CHA without BMSCs was implanted on another side as the control. All dogs were killed 16 weeks after implantation, and the periodontal tissue regeneration were observed histologically. Results：More new bone formation and more mature alveolar bone were observed in the test group than that in the control, and there was a clearly periodontal ligament-like tissue formation only in the test group. Conclusion-The complex of BMSC-root- CHA can improve the periodontal tissue regeneration.

关 键 词：骨髓基质细胞 牙周组织工程 珊瑚羟基磷灰石 体内再植 

分 类 号：R318.08[医药卫生—生物医学工程]