罗红霉素对人牙周膜成纤维细胞TNF-α、IL-6释放的影响  

Effects of roxithromycin on TNF-α and IL-6 release from periodontal ligment fibroblasts stimulated with lipopolysaccharide

作　　者：范永祥[1] 刘琪[1] 张绍伟[1] 葛正龙[2] 

机构地区：[1]贵州遵义医学院口腔医院 [2]遵义医学院生物化学教研室

出　　处：《实用口腔医学杂志》2006年第1期115-117,共3页Journal of Practical Stomatology

基　　金：贵州省教育厅基金资助项目;编号C-200;贵州省卫生厅优秀青年基金资助项目;编号D-164

摘　　要：目的：探讨罗红霉素对脂多糖诱导的人牙周膜成纤维细胞肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）释放的影响。方法：采用ELISA方法，检测在不同剂量的罗红霉素作用下，脂多糖诱导的人牙周膜细胞中IL-6、TNF-α释放的变化。结果：LPS刺激后人牙周膜细胞TNF-α的释放显著高于正常对照组（360pg／ml vs 80pg／ml，3 h，P〈0．05）。与L组相比，罗红霉素组（20μg／ml）TNF-α水平12h显著降低，3—6h与L组无明显差异，而12h（520pg／ml vs 710pg／ml）、24h（220pg／ml vs 680pg／ml）显著降低（P〈0．05）。LPS组IL-6水平显著高于正常对照组（360pg／ml vs 105pg／ml，P〈0．05），罗红霉素组（20μg／ml）IL-6分泌量在6h（420pg／ml vs 670pg／ml）、12h（590pg／ml vs 810pg／ml）和24h（340pg／ml vs 630pg／ml）较L组均显著降低（P〈0．05）。结论：罗红霉素能显著抑制LPS刺激后人牙周膜细胞IL-6、TNF-α的释放。Objective：To test the effects of roxithromycin（RM） on TNF-α and IL-6 release from periodontal ligment fibroblasts（PDLCs） stimulated with lipopolysaccharide（LPS）. Methods：PDLCs of passage 4 - 8 were cultured with RM at 2, 20 and 200 μg/ml respectively with 10 μg/ml of LPS. The control cells were cultured with culture medium only. The cultures were continued for 3,6,12 and 24 hours respectively. ELISA method was used to measure TNF-α and IL- 6 released into the cuhure medium from PDLCs in the different groups. Results：LPS increased both TNF-α and IL-6 release from PDLCs at all the time points（ P 〈 0.05 ）. RM inhibited the releases dosedependently（P 〈0.05 ）. Conclusion： RM may inhibit the secretion of TNF-α and IL-6 of PDLCs stimulated with LPS.

关 键 词：牙周炎 罗红霉素 内毒素 牙周膜成纤维细胞 肿瘤坏死因子-Α 白细胞介素-15 

分 类 号：R96[医药卫生—药理学]