人血清氧化低密度脂蛋白自身抗体的提取和初步鉴定  

Isolation and identification of human anti-oxidized LDL autoantibodies

作　　者：汪俊军[1] 朱雁飞[2] 强红娟[1] 张春妮[1] 

机构地区：[1]南京军区南京总医院全军医学检验中心,南京210002 [2]南京大学医学院

出　　处：《临床检验杂志》2006年第1期4-6,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(30471649);江苏省人类功能基因组学重点实验室开放课题(200040928)资助。

摘　　要：目的提取和鉴定人血清氧化低密度脂蛋白(Ox-LDL)自身抗体。方法采用溴化氰活化的Sepharose4B交联Ox-LDL制备免疫吸附层析柱,从正常人血清中提取抗Ox-LDL自身抗体,采用ELISA法鉴定其免疫反应性,散射免疫比浊法分析自身抗体种类。结果用NaHCO3、醋酸盐和KCNS溶液洗脱,分别收集洗脱峰。8份正常人血清中均收集到Ox-LDL自身抗体,与Ox-LDL均具有良好的反应性。自身抗体种类中以IgG为主,其次为IgM。结论此法提取的抗Ox-LDL自身抗体同Ox-LDL具有良好的反应能力。Objective To isolate and identify human anti-oxidized low density lipoprotein （LDL） autoantibodies. Methods Oxidized LDL （Ox-LDL） autoantibodies were isolated from eight healthy subjects by affinity chromatography with using an Ox-LDL cross-linked Sepharose 4B column. Results Three elution peaks of Ox-LDL autoantibodies were washed out from the Ox-LDL column by NaHCO3, acetate and KCNS buffer in turn and collected respectively. Ox-LDL autoantibodies were isolated from all the selected healthy subjects. All the three elution peaks had good reactivity with Ox-LDL. Among them,the elution peaks by KCNS buffer had high protein level and strong reactivity with Ox-LDL. The types of these antibodies included IgG and IgM, and IgG was the main type. Conclusion The isolated Ox- LDL autoantibodies had strong reactivity with Ox-LDL.

关 键 词：低密度脂蛋白 氧化修饰 自身抗体 动脉粥样硬化 

分 类 号：R446.1[医药卫生—诊断学]