我国主要地方绵羊品种mtDNA D-loop区PCR-RFLP研究  

Study on mtDNA D-loop of Chinese Main Indigenous Sheep Breeds Using PCR-RFLP

作　　者：李祥龙[1] 张增利[1] 巩元芳[2] 刘铮铸[2] 贾青[1] 王立泽[1] 

机构地区：[1]河北农业大学动物科技学院,保定071001 [2]河北科技师范学院动物科学系,昌黎066600

出　　处：《遗传》2006年第2期165-170,共6页Hereditas（Beijing)

基　　金：河北省自然科学基金资助项目(编号:302423)的部分内容~~

摘　　要：利用5种限制性内切酶(HinfⅠ,MspⅠ,Sau3AⅠ,XspⅠ,TaqⅠ),采用PCRRFLP技术研究了我国9个地方绵羊品种以及2个引入品种共计83只绵羊个体线粒体DNADloop区的多态性。结果表明,我国主要地方绵羊品种线粒体DNADloop区存在两种基本单体型,提示我国主要地方绵羊品种起源于两个母系祖先。线粒体DNADloop区多态度为0.0421%,说明我国地方绵羊品种线粒体DNA多态度较为贫乏。The polymorphism of mtDNA D-loop of 83 individuals from 9 Chinese indigenous sheep breeds and 2 imported sheep breeds were studied with 5 endonucleases, Hinf Ⅰ, Msp-Ⅰ, Sau3A Ⅰ, Xsp Ⅰ and TaqⅠ, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The averaged polyrnorphic degree （π value= 0. 0421% ） of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor.

关 键 词：地方绵羊品种 线粒体DNA D-LOOP PCR-RFLP 

分 类 号：S826.2[农业科学—畜牧学]