核糖体蛋白rpl15cDNA序列在真骨鱼类系统进化研究中的价值  

The Study of Ribosomal Protein L15 cDNA Sequences as a Molecular Marker in the Teleostei Phylogenetic Analysis

作　　者：向筑[1] 张竞男[1] 宋平[1] 胡珈瑞[2] 钟扬[3] 

机构地区：[1]武汉大学生命科学学院教育部发育生物学重点实验室,武汉430072 [2]武汉大学中南医院,武汉430071 [3]复旦大学生命科学学院生物多样性与生态工程教育部重点实验室,上海200433

出　　处：《遗传》2006年第2期171-178,共8页Hereditas（Beijing)

基　　金：国家自然科学基金项目(No.30150005和No.30270675);第三世界科学院研究基金项目(编号:95348RG/BIO/AS)资助~~

摘　　要：应用RTPCR,从真骨总目(Teleostei)5目15种鱼类中首次克隆了核糖体大亚基蛋白L15(rpl15,ribosomalproteinL15)的完整cDNA序列。以海鲢形亚组(Elopomorpha)的鳗鲡作为外类群,对这些真骨鱼类的核糖体蛋白L15cDNA序列进行了系统发育分析,结果表明:(1)rpl15基因在真骨鱼类等许多真核生物进化中高度保守;(2)系统树中各物种之间的关系与形态分类一致。rpl15编码区适合于真骨鱼类目以上分类阶元的分子系统学研究。In this study, the full-length cDNAs of rpl15 （ribosomal protein L15） gene were cloned and sequenced from fifteen fishes of five orders under Teleostei. The complete ORF sequences were analyzed for phylogenetic reconstruction for the first time to evaluate the potential of rpl 15 gene as a novel marker in resolving teleostean phylogenetic relationships. The resuttant NJ, MP and ML trees with Anguilla japonica as the outgroup were generatty resotved and largely congruent with the morphology-based tree though some incongruities were observed. The incongruities were then analyzed for some explanations. The results suggested that： （1） rpl 15 gene was highty conserved during eukaryotic evolution; （2） rpl 15 ORF might be a good phylogenetic marker for resolving teleoste an relationships. It might be especially appropriate for the higher-level relationships （such as interordinal）, and it was possibly suitable for lowerlevel relationships as well. The same can be true for other eukaryotes.

关 键 词：核糖体蛋白L15(rpl15) 分子系统学 真骨鱼类 

分 类 号：Q951.3[生物学—动物学]