盐酸阿比朵尔抗呼吸道合胞病毒机制初探  

Anti-viral Activity of Arbidol Hydrochloride Against Respiratory Syncytial Virus in Vitro

作　　者：何静[1] 杨占秋[1] 石丽桥[1] 刘媛媛[1] 肖红[1] 

机构地区：[1]武汉大学医学院病毒学研究所/病毒学国家重点实验室,武汉430071

出　　处：《武汉大学学报（医学版）》2006年第1期69-71,共3页Medical Journal of Wuhan University

摘　　要：目的:明确盐酸阿比朵尔抗吸道合胞病毒(RSV)Long株的活性并探讨其抗病毒机制。方法:通过细胞病变效应(CPE)、噻唑蓝(MTT)比色法检测细胞活性,观察盐酸阿比朵尔抗RSV的作用。结果:盐酸阿比朵尔的半数毒性浓度(TD50)为85.39 mg/L,当该药浓度为25 mg/L时,药物抗病毒生物合成组、药物直接作用组、药物抗吸附4 h组、药物抗病毒吸附8 h组的病毒抑制率分别能达到81.61%,79.57%,40.39%和78.60%。4组相应的药物半数有效浓度(ED50)为11.52,8.74,25.36和10.40 mg/L。结论:盐酸阿比朵尔在体外能明显抑制RSV引起的CPE效应,病毒抑制率随药物浓度的增加而增高,盐酸阿比朵尔能通过多种途径发挥抗RSV的作用。Objective： To study the antiviral activity of Arbidol hydrochloride on respiratory syncytial virus （RSV） Long strain in vitro. Methods： Anti-RSV effects were investigated by observing cytopathic effect （CPE） with MTT colorimetric assay. Results： The median cell inhibitory （TD500） of Arbidol was 85.39 mg/L and at its concentration of 25 mg/L, the viral inhibitory rates were up to 81.61%, 79.57%, 40. 39% and 78. 60% respectively in antiviral biological synthesis group, directly-killing-virus group and interfering-in-viral-absorption groups （pretreated cells with Arbidol for four and eight hours respe-ctively）. The corresponding median efficacious （ECs0） concentrations of Arbidol against RSV in the four groups were 11. 52, 8.74, 25.36 and 10. 40 mg/L, respectively. Conclusion： Arbidol could significantly inhibit cytopathic effect （CPE） of RSV-infected HEp-2 cells and increase survival rates of RSV-infected cells in a dose dependent manner, and could be used as an effective drug for RSV infection.

关 键 词：盐酸阿比朵尔 呼吸道合胞病毒 抗病毒作用 

分 类 号：R373[医药卫生—病原生物学]