祛障灵滴眼液对大鼠晶状体上皮细胞凋亡相关基因的调控  

Effect of quzhangling(祛障灵)on apoptosis for expression of rat lens epithelial cells for Bcl-2 and Bax

作　　者：张殷建[1] 阮雯洁[1] 刘莺[2] 吴中华[2] 

机构地区：[1]上海中医药大学附属龙华医院眼科,上海200032 [2]上海中医药大学实验中心

出　　处：《中国中医眼科杂志》2006年第1期38-40,共3页China Journal of Chinese Ophthalmology

基　　金：上海市卫生局中医临床专科紧缺人才培养基金[沪卫中医(2001)20]

摘　　要：目的探讨中药祛障灵滴眼液对氧化损伤大鼠晶状体上皮细胞(lens epithelial cells,LEC)凋亡相关基因Bcl-2和Bax的调控。方法用H2O2复制大鼠晶状体氧化损伤白内障离体模型,以吡诺克辛纳滴眼液为对照,设祛障灵临床剂量组和祛障灵高浓度组。观察祛障灵滴眼液对氧化损伤大鼠LEC凋亡相关基因Bcl-2和Bax的调控。结果①正常大鼠LEC中Bcl-2和Bax均有表达,Bcl-2较Bax表达强。②H2O2组Bcl-2表达显著下调,Bax表达显著上调。Bcl-2/Bax比率下降。③与H2O2组比较,药物组可明显上调Bcl-2表达,下调Bax表达。Bcl-2/Bax比率上升。结论中药祛障灵滴眼液能够调控氧化损伤大鼠晶状体上皮细胞凋亡相关基因Bcl-2和bax的表达。具有对晶状体的抗氧化损伤作用。OBJECTIVE To investigate effect of quzhangling （祛障灵） on apoptosis for expression of rat lens epithelial cells for Bcl- 2 and Bax in rat lens epithelial cells （LEC） injured by oxidation.METHODS The model of oxidative damages were established in lens of rats and they were divided randomly into control group, Hydrogen peroxide group （H202）, pirenoxine sodium group, quzhangling sodium, low dosage group and high dosage group. The expression of Bcl - 2 and Bax protein of LEC were measured and compared by tearing the LEC anterior capsule via immunohistochemical analysis. RESULTS There were Bcl - 2 and Bax expression in normal lenses of SD rates, Bcl - 2 expression was stronger than Bax. Bcl - 2 expression decreased and Bax expression increased markedly, Bcl - 2/Bax reduced in H202 group. There were up regulation of Bcl - 2 expression and down regulation of Bax expression, and Bcl - 2/Bax increased in quzhangling group compared with H202 group group and quzhangling sodium group. CONCLUSIONS apoptosis- related genes, Bcl- 2 and Bax, which may quzhangling. The above changes were similar between pirenoxine sodium These results show that quzhangling regulates expression of be the molecular mechanism of LEC apoptosis inhibition by quzhangling.

关 键 词：祛障灵滴眼液 白内障 细胞凋亡 基因表达 

分 类 号：R285.5[医药卫生—中药学]