提高甘蓝型油菜下胚轴外植体植株再生的研究  

Studies on the enhancement of plant regeneration from hypocotyl explants of Brassica napus.

作　　者：何云[1] 万光龙[1] 唐桂香[1] 许玲[1] 周伟军[1] 

机构地区：[1]浙江大学农学系,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2006年第1期46-50,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省科技厅重点项目(2004C220132005C22004);浙江省自然科学基金(Y304162)

摘　　要：以甘蓝型油菜品种N1、N2、N3、N4、N5和N6的下胚轴为材料,研究外植体芽再生的影响因素.结果表明:不同植物激素的组合、Ag+、蔗糖浓度、GA3、外植体的生理状态即苗龄以及基因型对外植体的再生均有较大影响.4 d苗龄的下胚轴置于含1.5 mg?L-12,4-D的MS培养基中预培养7 d,再转接到含30 g?L-1蔗糖的分化培养基(MS+BA 4 mg?L-1+NAA 0.1 mg?L-1+AgNO32.5 mg?L-1)上,芽再生频率和再生外植体数均较高,其中品种N1分别达到了40%和3.7.添加1.0 mg?L-1GA3可促进胚状体形成,芽苗再生频率显著提高(品种N1达到了95%,比不添加GA3提高了1倍多),但较易形成玻璃苗.Effects of various hormones, Ag^＋, sucrose concentration, GA3, seedling age and genotypes on shoot regeneration from hypocotyl segments of 6 varieties of Brassica napus were examined in vitro. Results showed that all these factors had great influence. Hypocotyl explants of 4-day-old seedlings showed high regeneration frequency and number of shoots per explant （40% and 3.7 in cv. N1 ）, which were pre-cultured for 7 days in a MS medium with 1.5 mg ·L^-1 2, 4-D and then sub-cultured in differentiation medium of MS＋4 mg·L^-1 BA＋0.1 mg·L^-1 NAA＋2.5 mg·L^-1 AgNO3 supplemented with 30 g·L^-1 sucrose. Addition of 1.0 mg·L^-1 GA3 in media could promote the formation of embryos and improve shoot regeneration frequency remarkably （reached 95% in cv. N1 ）. Vitrification of the plantlets was, however, more liable to occur under such condition.

关 键 词：甘蓝型油菜 下胚轴 AG^+ GA3 基因型 离体再生 

分 类 号：S565.403[农业科学—作物学]