Gene expression profiling under different photoperiod/temperature conditions in a photoperiod-/thermo-sensitive genic male sterile line of rice(Oryza sativa L.)  

作　　者：GUO Xiaoqin DONG Haitao ZHENG Kangle LUO Hongmei TAN Xuelin FANG Yongqi WANG Yanqin DENG Ye DAI Chengen LOU Yichun SHAO Jing SHI Wenqin ZHAO Dong LI Debao 

机构地区：[1]Bioinformatics and Gene Network Research Group, Zhejiang University, Hangzhou 310029, China [2]National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China [3]College of Life Sciences, Hangzhou Teachers College, Hangzhou 310012, China [4]Rice Research Institute, Yunnan Agricultural University, Kunming 650201, China

出　　处：《Chinese Science Bulletin》2006年第2期175-181,共7页

摘　　要：Peiai 64S is a photoperiod- and thermo- sensitive genic male sterile (PTSGMS) line of rice, which is male sterile at long day/high temperature and partial fertile at short day/low temperature. A cDNA array representing 3328 unique rice genes was used to profile the gene expression patterns in the young panicles of Peiai 64S under these two condi- tions. The statistical data showed that up to 14.60% of genes exhibited up-or down-regulated expressions in the plants at long day/high temperature compared with plants at short day/low temperature. Only four genes were up-regulated while 482 genes down-regulated. Real-time PCR with all the up-regulated and 9 randomly selected down-regulated genes confirmed the differential expressions detected by the array, indicating that the constructed cDNA array is reliable. These differently expressed genes participated in almost all cell biological responses. Analysis of up- and down-regulated genes revealed distinctive changes between the mRNA abundances of MMK1 and MMK2, both of which are analogs ofMAPK, and significant down-regulation of several transcription factors. It was suggested that changes that occurred in the MAPK signal transduction path- way might disturb the transcription control necessary for morphogenesis of pollens and corresponding phys- iological functions.Peiai 64S is a photoperiod- and thermosensitive genic male sterile （PTSGMS） line of rice, which is male sterile at long day/high temperature and partial fertile at short day/low temperature. A cDNA array representing 3328 unique rice genes was used to profile the gene expression patterns in the young panicles of Peiai 64S under these two conditions. The statistical data showed that up to 14.60% of genes exhibited up-or down-regulated expressions in the plants at long day/high temperature compared with plants at short day/low temperature. Only four genes were up-regulated while 482 genes down-regulated. Real-time PCR with all the up-regulated and 9 randomly selected down-regulated genes confirmed the differential expressions detected by the array, indicating that the constructed cDNA array is reliable. These differently expressed genes participated in almost all cell biological responses. Analysis of up- and down-regulated genes revealed distinctive changes between the mRNA abundances of MMK1 and MMK2, both of which are analogs of MAPK, and significant down-regulation of several transcription factors. It was suggested that changes that occurred in the MAPK signal transduction pathway might disturb the transcription control necessary for morphogenesis of pollens and corresponding phys- iological functions.

关 键 词：水稻 光周期 温度 热敏感性 雄性不育基因 基因表达 信号转导 

分 类 号：S511[农业科学—作物学] Q786[生物学—分子生物学]