辽宁碱蓬甜菜碱醛脱氢酶基因(BADH)启动子分离及序列分析  

Isolation of BADH Gene Promoter from Suaeda liaotungensis and its Sequence Analysis

作　　者：李秋莉[1] 张毅[1] 尹辉[1] 李丹[1] 

机构地区：[1]辽宁师范大学生命科学学院,大连116029

出　　处：《生物工程学报》2006年第1期77-81,共5页Chinese Journal of Biotechnology

基　　金：辽宁省科技基金资助项目(No.20031060);辽宁省教育厅资助项目(No.2004F091)~~

摘　　要：根据已知的辽宁碱蓬BADHcDNA5′端序列设计两个基因特异的反向引物(BR1,BR2),通过衔接头PCR获得了BADH基因起始密码子上游265bp的序列。根据所获得的序列设计两个基因特异的反向引物(BR3,BR4),用BR2、BR3、BR4分别与4个简并引物配对,通过TAILPCR扩增,获得了约2kb的序列。经Sequencer软件拼接上述两段序列,获得了BADH基因起始密码子上游2055bp的序列。用TSSPTCM软件分析此序列,预测出转录起始点(T)位于起始密码子上游62bp处,由此获得了1993bp的SlBADH启动子序列。用PLACE软件分析此序列,发现该序列具有启动子的基本元件TATAbox、CAATbox,包含多个胁迫诱导元件,如盐诱导元件GAAAAA,抗冻、缺水、脱落酸、抗寒元件CANNTG,伤害诱导元件ANATTNCNN,热激元件ATAAATGT等,是一个强的胁迫诱导启动子。In this study, the 5＇-flanking proximal region of stress-induced gene encoding betaine aldehyde dehydrogenase was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis. 1993 bp sequence was obtained by sequencing. The transcription start site, which localized at 62 bases upstream of the start ATG, was predicted using TSSP-TCM program. The functional elements were analysed by PLACE programm. The SlBADH gene promoter contains the basic elements： TATA-box, CAAT-box, and stress-induced elements： salt responsed element, cold, dehydration, ABA and frozen responsed elements, WUN responsed elements and HSE. Obtaining the promoter of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis provides a foundation for analyzing the stress-induced promoter elements, studying the relationship between structure and founction of the promoter, and investigating the molecular mechanism of BADH gene regulation.

关 键 词：辽宁碱蓬 甜菜碱醛脱氢酶 启动子 序列分析 

分 类 号：Q943.2[生物学—植物学]