东北虎幼体消化系统蛋白水解酶的初步研究  

Preliminary studies on proteolytic enzymes in the digestive system of Amur tiger cubs(Panthera tigris altaica)

作　　者：牛红星[1] 卜艳珍[1] 余燕[2] 姬生栋[2] 王艳梅[2] 卢全伟[2] 刘敬泽[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016 [2]河南师范大学生命科学学院

出　　处：《兽类学报》2006年第1期98-100,共3页Acta Theriologica Sinica

基　　金：河南省自然科学基金资助项目(0411032500);河南省动物学省级重点学科经费资助

摘　　要：The kinds and activities of proteolytic enzymes in the digestive systems (tongue, esophagus, stomach, duodenum, liver, pancreas and large intestine) of two Amur tiger cubs (Panthera tigris altaica) were investigated using protein detection and SDS-G-PAGE. The results showed:1) the digestive system of Amur tiger cubs had 30 different kinds of proteolytic enzymes ; 2) the mostly optimal pH for these enzymes was a neutral environment, and an acid condition highly restrained their activity; 3) the proteolytic enzymes in the tongue and esophagus were fewer than in other digestive organs and their activities were weak as well; 4) the duodenum had the most kinds of proteolytic enzymes with the strongest activities under various pH conditions; 5) the 16 kD enzyme bands always existed under neutral and alkalescence pH conditions in all digestive organs except the tongue.The kinds and activities of proteolytic enzymes in the digestive systems （ tongue, esophagus, stomach, duodenum, liver, pancreas and large intestine） of two Amur tiger cubs （ Panthera tigris altaica） were investigated using protein detection and SDS-G-PAGE. The results showed ： 1 ） the digestive system of Amur tiger cubs had 30 different kinds of proteolytic enzymes ; 2 ） the mostly optimal pH for these enzymes was a neutral environment, and an acid condition highly restrained their activity ; 3 ） the proteolytic enzymes in the tongue and esophagus were fewer than in other digestive organs and their activities were weak as well ; 4 ） the duodenum had the most kinds of proteolytic enzymes with the strongest activities under various pH conditions; 5） the 16 kD enzyme bands always existed under neutral and alkalescence pH conditions in all digestive organs except the tongue.

关 键 词：东北虎幼体 消化系统 蛋白水解酶 复性电泳 

分 类 号：Q556.3[生物学—生物化学]