庆大霉素损伤小鼠前庭的实验模型  

An Experimental Model of Vestibular Dysfunction by Injecting Gentamicin in Mice

作　　者：栗志远[1] 黄矛[1] 张晓冬[1] 佘佳红[1] 陆国才[1] 

机构地区：[1]第二军医大学基础部新药评价中心,上海200433

出　　处：《癌变．畸变．突变》2006年第1期1-5,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(No.30471932);第二军医学青年启动基金(No;2003SQ001)

摘　　要：背景与目的建立一种操作简单、切实可行的前庭损伤小鼠模型。材料和方法小鼠皮下注射(Subcutaneous,sc)庆大霉素400mg/(kg·d)连续15d,每天两次。停药后12h以接触翻正反射恢复时间,头偏和游泳行为对小鼠前庭功能作评估并处死取前庭终器作病理学组织观察。结果庆大霉素400mg/(kg·d)组小鼠在最后一次给药后12h,接触翻正反射恢复时间明显延长,头偏明显且游泳能力显著降低,组织病理学检查证实前庭器的位觉斑和壶腹嵴出现明显损伤。结论该模型是一种操作简单、切实可行的前庭损伤小动物模型。BACKGROUND＆AIM： To establish a convenient and feasible animal model of vestibular dysfunction in mice. MATERIAL AND METHODS： A dose of 400 mg gentamicin /（kg · d） was given to mice subcutaneously twice daily for 15 consecutive days. Vestibular function was assessed by contact righting reflex as well as the head tilt and swimming behavior, and histopathology of the animals＇ saccular and utricular macula or crista ampullaris was examined. RESULTS： Time of contact righting reflex was prolonged, scores of head tilt and swimming behavior were higher than control. Meanwhile, damage of saccular and utricular macula or crista ampullaris of mice were confirmed by histopathological examination. CONCLUSION： This is a convenient and feasible model of experimental vestibular dysfunction in small animals for research on pharmacodynamics of prophylactic drugs for motion sickness and vertigo.

关 键 词：前庭损伤 庆大霉素 小鼠 模型 接触翻正反射 头偏 

分 类 号：R965.1[医药卫生—药理学] R595.3[医药卫生—药学]