大黄制剂对小鼠的急性毒性和自然免疫调节作用  

Rhubarb Acute Toxicity and Innate Immune Modulation in Mice

作　　者：孙丽霞[1] 任金荣[1] 单保恩[1] 李元振[1] 何兰欣[1] 艾军[1] 

机构地区：[1]河北医科大学第四医院河北省肿瘤研究所免疫室,河北石家庄050011

出　　处：《癌变．畸变．突变》2006年第1期35-37,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河北省卫生厅科研基金项目(No.04241)

摘　　要：背景与目的探讨大黄煎剂对小鼠的急性毒性和自然免疫功能的影响。材料与方法经LD50预试验后,根据LD50试验的结果取健康昆明种小鼠40只,设置0.9%盐水阴性对照组及3个不同剂量土大黄煎剂组(5000、2500、1000mg/kg),按,1次/d给药,连续灌胃10d后,分别测定小鼠的外周静脉血红细胞免疫黏附活性、NK细胞的杀伤活性、补体的总活性及脾细胞的增殖反应。结果小鼠对大黄煎剂的LD50为8043mg/kg。不同剂量药物连续灌胃10d后,可明显提高小鼠NK细胞的杀伤活性,与盐水对照组相比差别有统计学意义(P<0.05);并能增强PHA诱导的脾细胞增殖反应能力,随药物的剂量增大,增殖反应能力越增强;5000mg/kg和2500mg/kg两个剂量组与对照组相比,补体总活性较盐水对照组降低,红细胞免疫黏附功能增强,差别有统计学意义(P<0.05)。结论大黄煎剂具有提高细胞免疫,对正常的免疫细胞具有促进增殖作用;能够激活NK细胞,提高NK细胞杀伤活性;降低补体总活性,增强红细胞自然免疫的作用。以上表明大黄煎剂对特异性免疫、非特异性免疫均有促进作用,可辅助其它药物进行临床治疗。BACKGROUND ＆ AIM： This study was designed to elucidate the acute toxicity and immune modulation of Rhubarb concoction in mice. MATERIAL AND METHODS： After LD50 test,the mice were randomly divided into four groups： the control, 1 000 mg/kg,2 500 mg/kg,5000 mg/kg, given as 0.01-0.02 ml/g,daily for 10 days.The complement activities and erythrocyte CR1 adherence function were measured by using trace fast plate complement activity assay and complement activating yeast rosette test, respectively.NK cell activities were measured by improved MTT method. RESULTS： LDs0 was about 8 043 mg/kg. Rhubarb enhanced NK cell activities and erythrocyte CR1 adherence function and decreased complement activity. CONCLUSION： Rhubarb could enhance cell immunity and promote immune cell proliferation.h stimulated NK cell activities and Erythrocyte CR1 adherence activity.h could be used as an adjuvant therapy.

关 键 词：大黄煎剂 补体 红细胞免疫 NK细胞 

分 类 号：R285.5[医药卫生—中药学]