心肌梗死后心力衰竭大鼠空间学习和记忆能力的变化(英文)  

Changes of spatial learning and memory ability in rats with heart failure after myocardial infarction

作　　者：秦英[1] 王硕仁[1] 王伟东[1] 赵明镜[1] 吕浠莹[1] 朱陵群[1] 

机构地区：[1]北京中医药大学东直门医院教育部中医内科学重点实验室暨北京市中医内科学重点实验室,北京市100700

出　　处：《中国临床康复》2006年第2期172-175,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金项目(A3017118);国家人事部博士后科学基金资助项目(中博基200114);北京市科技新星计划资助项目(H020820860130)~~

摘　　要：背景:慢性充血性心力衰竭是许多心脏疾病的晚期表现,大多数临床研究报道支持充血性心力衰竭时存在认知障碍,而成功的心功能改善措施具有提高认知功能的作用。目的:探讨心肌梗死后充血性心力衰竭对大鼠空间学习和记忆能力的影响,观察心力衰竭与认知功能之间的相关性。设计:以实验动物为观察对象的随机对照观察。单位:北京中医药大学东直门医院教育部中医内科学重点实验室暨北京市中医内科学重点实验室。材料:实验于2001-11在北京中医药大学东直门医院教育部(北京市)重点实验室完成。选择购于北京维通利华实验动物技术有限公司的10～12周龄健康雄性SD大鼠100只,体质量(200～220)g,随机分为手术组(n=60)和假手术组(n=40)。方法:手术组大鼠采用结扎左冠状动脉前降支的方法制作急性心肌梗死模型,假手术组大鼠在左冠状动脉前降支留置一松结,不结扎。将心电图示8个导联有心肌梗死样改变的存活大鼠作为符合条件的手术组大鼠(n=33),心电图没有异常改变的未结扎存活大鼠作为符合条件的假手术组大鼠(n=25),将两组分别按时间分为10d组、30d组、60d组。分别于术后10、30、60d进行Morris水迷宫实验,并测血流动力学指标。在手术10d组、30d组和60d组中,由于心力衰竭(简称心衰)体力不济而无法完成全部Morris游泳实验,且在实验后2d内死亡或血流动力学测定后死亡的大鼠组成心衰终末期组,其对照组为与死亡大鼠同组的体质量相近的手术组大鼠。主要观察指标:①逃避对数潜伏期和认知能力较好率。②每搏量、每分心排出量、心率和心指数值,其中心指数值为主要反应心功能的指标。结果:符合条件的手术组大鼠33只,假手术组大鼠25只,进入结果分析。①血流动力学指标测定结果:手术10d、30d、60d组大鼠的每搏量、每分心排出量、心指数均明显低于同期假BACKGROUND： Chronic congestive heart failure occurs at the late stage of many heart diseases. Cognitive disability exists in chronic congestive heart failure which has been reported in most clinical studies, and measures that can successfully improve heart function can improve cognitive function. OBJECTIVE： To probe into the effect of chronic congestive heart failure after myocardial infarction on spatial learning and memory ability, and to observe the correlation between heart failure and cognitive func- DESIGN： A randomized controlled observation based on the experimental animals. SETTING： Key Laboratory of Traditional Chinese Medicine （Beijing University of Chinese Medicine）, Ministry of Education. MATERIALS： This experiment was performed at the Key Laboratory of Traditional Chinese Medicine （Beijing University of Chinese Medicine）, Ministry of Education. in November 2001. Totally 100 healthy male rats aged 10 to 12 weeks, weighing 200 g to 220 g purchased from Beijing Weitong Lihua Experimental animal Technical Co.Ltd were enrolled and randomly divided into the operation group （n=60） and sham-operation group （n=40）. METHODS： Model rats in the operation group were infarcted by ligation of the left anterior descending coronary arteries to produce acute myocardial infarction models. Those in the sham-operation group left a loose tie without ligation. The rats that showed myocardial infarction-like changes through electrocardiogram were taken as the rats for operation （n=38）, and those survived rats without abnormal changes and without ligation were taken as the sham-operation group （n=25）. The two groups were subdivided into 10-day group, 30-day group and 60-day group. Morris water maze test was performed 10, 30 and 60 clays after the operation followed by hemodynamic monitoring. Among the 10-day group, 30-day group hnd 60-day group, rats that did not complete the whole Morris swimming test due to heart failure with poor power and died 2 clays after the operation or after hemo

关 键 词：心力衰竭 充血性 学习 记忆 大鼠 Sprague—Dawley 

分 类 号：R338.64[医药卫生—人体生理学]