人肝细胞刺激因子对环磷酰胺致S_(180)荷瘤小鼠肝损伤的保护作用  

Protection of hepatic stimulator substance against chemotherapeutic liver damage induced by cyclophosphamide in mice inoculated with S_(180) cells

作　　者：姚琦[1] 倪秀雄[1] 陈炜[1] 

机构地区：[1]福建医科大学生理学与病理生理学系

出　　处：《中国临床药理学与治疗学》2006年第1期82-85,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:肝细胞刺激因子(HSS)对环磷酰胺(CP)致S180荷瘤小鼠化疗性肝损伤的保护作用及可能机理。方法:采用S180荷瘤小鼠,观察HSS对CP化疗后S180荷瘤小鼠血清生化指标和肝组织匀浆氧化及抗氧化指标、肝组织病理学以及S180瘤重的影响。结果:HSS明显降低CP化疗组S180荷瘤小鼠肝组织丙二醛(MDA)含量,使肝组织谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽-S-转移酶(GST)和谷胱甘肽过氧化物酶(GSH-PX)活性升高;血清谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)活性,HSS治疗组与对照组、CP化疗组比较均无显著性差异;病理学检查发现CP化疗组肝组织点状及小灶性坏死,坏死区及汇管区大量炎性细胞浸润,HSS可部分逆转这些变化;HSS治疗组瘤重与CP化疗组瘤重无显著性差异。结论:CP可致S180荷瘤小鼠化疗性肝损伤,HSS具有保护作用,其抗损伤机制可能与抗氧化应激有关。AIM： To study the protective effect of hepatic stimulator substance （HSS） on chemotherapeutic liver damage induced by cyclophosphamide and its possible mechanism. METHODS： The serum biochemical parameters, oxidative parameters and anti-oxidative parameters of liver tissue of male Kunming mice inoculated with S180 cells were measured. The pathology of the hver was observed with microscope. The weight of S180 sarcom was weighted. RESULTS： The elevation of MDA content of liver tissue and the less of GSH content and CAT, GSH-PX, GST, and SOD activities induced by CP were restored remarkably by HSS. The ALT and LDH activities of serum in HSS group which was compared with control group and CP group were not statistically distinctive. In the pathologic examination, spotty and focal necroses of hepatocytes in the liver of CP group were found with a great deal of inflammatory cells infiltration in the necrotic and portal areas, while the damage in the liver of HSS group was ameliorated obviously; The weight of S180 sarcom in CP group and HSS group was not statistically distinctive. CONCLUSION： The effect of HSS on CP-induced chemotherapeutic liver damage maybe due to antioxidative activity.

关 键 词：环磷酰胺 化疗性肝损伤 肝细胞刺激因子 氧化应激 肝保护 

分 类 号：R965.1[医药卫生—药理学]