肾病综合征鼠骨代谢变化特点及芪归合剂对其的作用  

Change of Bone Metabolism in Rats with Nephrotic Syndrome and the Role of Astragalus and Angelica Mixture

作　　者：蒋小云[1] 陈述枚[1] 黄绍良[2] 莫樱[1] 赖峰[1] 朱志红[1] 

机构地区：[1]中山大学附属第一医院儿科,广东广州510080 [2]中山大学附属第二医院儿科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2006年第1期67-71,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省卫生厅科研基金资助项目(A2003168);广东省自然科学基金博士启动基金资助项目(04300346)

摘　　要：目的探讨肾病综合征(NS)鼠骨代谢的变化特点及芪归合剂对其的作用。方法制备大鼠阿霉素NS模型,设模型组、激素治疗组、芪归治疗组、激素芪归治疗组及正常对照组,每组8只,用药时间为3周。测量各组尿蛋白、血生化指标和右股骨长度;用HologicQDR-2000+DEXA行股骨全段骨密度(BMD)测定;以放射免疫法测定血清骨钙素(OC)、I型前胶原氨基端伸展肽(PINP)、I型胶原羧基端关联肽原(ICTP)水平。结果模型组股骨长度(3.35±0.09)cm,低于正常组(3.53±0.11)cm(P<0.05);芪归治疗组(3.46±0.09)cm,高于模型组(P<0.05);激素芪归组(3.28±0.07)cm,高于激素治疗组(3.16±0.11)cm(P<0.01);激素治疗组低于模型组(P<0.05)。模型组股骨全段BMD(0.1357±0.0137)g/㎝2,低于正常组(0.1599±0.0101)g/㎝2(P<0.01);激素治疗组(0.1208±0.0123)g/㎝2,低于模型组(P<0.05)。模型组血OC(16.31±3.87)μg/L和PINP(2.02±0.51)μg/L水平,低于正常组,分别(31.49±4.16)μg/L、(3.58±0.46)μg/L(P<0.01);芪归治疗组血OC(25.90±8.26)μg/L和PINP(3.42±1.07)μg/L水平,高于模型组(分别P<0.05和P<0.01);激素芪归组血OC(12.01±1.97)μg/L和PINP(2.47±0.88)μg/L水平,高于激素治疗组,分别(6.12±3.26)μg/L、(1.63±0.74)μg/L(P<0.05);激素治疗组低于模型组(P<0.05)。激素治疗组的ICTP(10.15±3.77)μg/L高于模型组(8.71±3.29)μg/L(P<0.05)。结论NS鼠存在骨代谢的异常,激素治疗使骨代谢异常进一步加剧,而芪归合剂治疗对其有改善作用。[Objective] To investigate the change of bone metabolism in rats with nephrotic syndrome （NS） and the role of Astragalus and Angelica mixture （A＆A）. [ Methods] Forty male SD rats （six weeks old） were randomly divided into five groups： control group, NS model group, NS group treated with A＆A, NS group treated with dexamethasone （Dex）, NS group treated with A＆A and Dex. Urine protein, serum biochemical levels and the femur length were measured. The bone mineral density （BMD） of whole part of femur was measured by Hologic ODR-2000＋DEXA. Serum osteocalcin （OC）, aminoterminal propeptide of type Ⅰ procollagen （PINP） and carboxytenninal telopeptide of type Ⅰ collagen （ICTP） levels were assayed by radioimmunoassay technique. [Results] The length of femur of NS group treated with A＆A and NS group treated with A＆A and Dex were higher than those in NS model group and NS group treated with Dex, respectively （P 〈 0.05 and P 〈 0.05）. The length of femur of NS group treated with Dex was lower than that of NS model group （P 〈 0.05）. The length of femur of NS model group was lower than that of control group （P 〈 0.05）. The BMD of femur in NS model group was lower than that of control group （P 〈 0.01）. The serum levels of OC and PINP in NS model group were lower than those in control group （P 〈 0.01）. The serum levels of OC and PINT in NS group treated with A＆A and NS group treated with A＆A and Dex were higher than those in NS model group and NS group treated with Dex, respectively （P 〈 0.05 and P 〈 0.01,respectively）. The serum levels of OC and PINP in NS group treated with Dex were lower than those in NS model group （P 〈 0.05）. The serum levels of ICTP in NS group treated with Dex were higher than that of NS model group （P 〈 0.05）.[Conclusion] The abnormality of bone metabolism exists in nephrotic rats. Dexamethasone treatment can aggravate this abnormality, which can be improved by Astragalus and Angelica mixture.

关 键 词：肾病综合征 骨代谢 黄芪 当归 

分 类 号：R692[医药卫生—泌尿科学]