水稻Rim2/Hipa超级家族的基因组变异和基于Rim2/Hipa展示的水稻资源的系统进化和指纹分析(英文)  

Genomic Variation of The Rice Rim2/Hipa Superfamily and Dendrogram and Fingerprinting Analysis of Rice Germplasm Based on Rim2/Hipa Paralog Display

作　　者：田平芳[1,4] 王建军[2] 吴刚[1] 李群[1] 卢宝荣[3] 何祖华[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,植物分子生物遗传国家重点实验室 [2]浙江省农业科学院作物与核技术利用研究所 [3]复旦大学生物多样性研究所 [4]北京化工大学生命科学与技术学院,北京100029

出　　处：《生物化学与生物物理进展》2006年第2期169-177,共9页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(90208010,30125030).~~

摘　　要：水稻Rim2/Hipa是最近鉴定的一个受逆境诱导的转座因子超级家族.研究表明,Rim2的核心序列在不同来源的水稻材料中存在显著的差异,暗示Rim2家族的长期进化历程.基于Rim2因子间的差异性以及该因子的静止状态,开发出一种利用Rim2因子展示的新的分子指纹技术,可以灵敏地区分不同水稻资源以及它们的遗传关系.仅用5对引物就可以清楚地将53个栽培稻和普通野生稻材料鉴定出来,并可将它们分为不同的系统进化组.研究表明不仅在水稻资源而且在野生稻种质间均存在明显的多样性.野生稻可以被单独分组,或者分散在粳稻中间.这种新的指纹技术还可以将水稻的杂交子代和它们的亲本区分出来,并可用于种子纯度的鉴定,在水稻基因组进化研究、水稻育种和种子生产中有很好的应用前景.The rice Rim2/Hipa is a stress-induced transposon superfamily recently identified in Oryza genomes. Genomic variation was found in the Rim2 core region among rice genetic resources/genomes, indicative of high genomic divergence accumulated during the Rim2 evolution. Based on the divergence and quiescent state of the Rim2 elements, a Rim2 paralog display-based fingerprinting approach was developed to effectively identify rice genetic resources and explore their genetic relationships within a set of rice germplasm including 45 accessions of O. sotiva and 8 accessions of its wild relatives O. rufipogon. A dendrogram showed not only clear genetic diversity of rice germplasm, but also considerable genetic differentiation among wild rice resources. The wild rice relatives were either clustered as an independent group, or among the japonica varieties. This Rim2-based fingerprinting approach could also serve as a sensitive tool to identify rice hybrids from their parents, and variety stability, demonstrating its great potential in evolution study of rice genomes and in rice breeding and seed production.

关 键 词：水稻 Rim2/Hipa 基因组差异 系统树 指纹 

分 类 号：Q74[生物学—分子生物学]