IgG-Eu-IDPA对抗原IOV的时间分辨荧光免疫检测  

Use IgG-Eu-IDPA to Detect Antigen IOV by Time Resolved Fluoroimmunoassay

作　　者：李占东[1] 吕会田[2] 乐加昌[1] 范青华[3] 李文[4] 罗志勇[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101 [2]北京工商大学生物系,北京100037 [3]中国科学院化学研究所,北京100080 [4]中国科学院理化技术研究所,北京100101

出　　处：《生物化学与生物物理进展》2006年第2期190-195,共6页Progress In Biochemistry and Biophysics

基　　金：国家高技术研究发展计划(863)资助项目(2002AA248051);国家自然科学基金资助项目(30100040).~~

摘　　要：镧系螯合物已经被广泛应用于高灵敏度的时间分辨荧光免疫分析.但是,使用紫外光激发镧系螯合物会对生物分子和细胞产生很大的损害.合成了能够被可见光(最大波长565nm)激发的IgG-Eu-IDPA,并测量了IgG-Eu-IDPA的光谱属性,如荧光寿命、在不同pH值和不同浓度环境下的荧光强度.在自制的时间分辨荧光仪上使用IgG-Eu-IDPA作为荧光探针,检测IOV抗原.数据显示,它的灵敏度远远高于传统的荧光仪.结果表明,IgG-Eu-IDPA能够在高灵敏度的原位和活体分析中作为一种新的、有潜力的荧光探针.Lanthanide chelates have been successfully developed as fluorescence probes and widely used for highly sensitive time resolved fluoroimmunoassay. However, Lanthanide chelates are excited by UV-light which do a lot of damage to the biomolecules and cells. IgG-Eu-IDPA which can be excited by visible light （the maximum excite wavelength is 565mn） was compounded. The other spectra properties such as fluorescence lifetime, fluorescence intensity at different pH, fluorescence intensity in different concentration were measured. It was used as a fluorescence probe to detect antigen IOV with the self-built time resolved luminoscope. Its sensitivity is much higher than that of ordinary luminoscope. The results indicate that IgG-Eu-IDPA can be a new potential fluorescence probe for high sensitivity in situ and in vivo analysis.

关 键 词：荧光光谱 荧光探针 时间分辨荧光免疫分析 

分 类 号：Q6[生物学—生物物理学]