单一剂量单侧腮腺放射损伤对双侧腮腺结构和功能的影响  

Effect of Single Dose Irradiation to Parotid Gland on the Structured and Function Changes of Bilateral Parotid Glands in Miniature Pig

作　　者：单兆臣[1] 李钧[1] 欧广飞[2] 刘晓勇[1] 张春梅[1] 王松灵[1] 

机构地区：[1]首都医科大学北京口腔医学院基因治疗分子生物实验室,北京100050 [2]北京协和医科大学中国医学科学院肿瘤医院放疗科,北京100021

出　　处：《华西口腔医学杂志》2006年第1期83-85,88,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30271400);国家杰出青年科学基金资助项目(30125042)

摘　　要：目的研究单一大剂量射线照射单侧小型猪腮腺对双侧腮腺结构和功能影响。方法14只小型猪一侧腮腺用直线加速分别给予15G(y7只)和20G(y7只)离子射线照射,4只做为空白对照。分别在放射前,放射后4周和16周观察腮腺唾液流率、腺体重量、腺泡面积和组织学变化。结果4周时,15Gy和20Gy照射后放射侧腮腺重量下降达50%;15Gy照射后放射侧腮腺唾液流率无明显下降,20Gy照射后放射侧腮腺唾液流率减少约50%。16周时,15Gy和20Gy照射后放射侧腮腺重量下降达50%,组织学明显改变,照射后放射侧腮腺流率分别下降约60%及80%。非放射侧腮腺重量及形态均无明显变化,但20Gy照射后16周时非放射侧腮腺唾液流率明显下降。结论单一剂量照射后腮腺结构的改变相对唾液流率下降发生较早,唾液流率减少与腺泡面积的减少不完全成正相关。非照射侧腮腺形态变化不明显,但唾液流率明显下降。Objective To evaluate the effects of a solitary megadose protocol of ionizing radiation （IR）to parotid gland on the structured and function changes of bilateral parotid glands in miniature pig. Methods Fourteen minipigs were subjected to either 15 or 20 Gy to one parotid gland with a linear accelerator, while another four minipigs served as non-IR controls. Salivary flow rates and salivary chemistries were measured pre-IR, and 4 and 16 weeks post-IR. A quantitative assessment of gland weight and acinar area, and detailed serum chemistry and hematological analyses, were also performed. Results Parotid gland weights were significantly decreased in the 15 and 20 Gy groups at 4 and 16 weeks post-IR. The acinar cell area in glands of both IR groups was significantly reduced. Parotid flow rates decreased by 600/o with 15 Gy at 16 weeks post-IR. In the 20 Gy group, salivary flow rates were reduced by 80% at 16 weeks post-IR. Additionally, parotid flow rates significantly reduced in contralateral glands with 20 Gy at 16 weeks, while structure and weight did not changes in parotid glands. Conclusion Structural changes in salivary gland parenchyma occurred relatively early after IR, while the alterations in salivary output were relatively delayed. Further, reductions in salivary flow were not proportional to acinar cell area loss. There isn＇t a significant structured change of contralateral glands, but significant reduction of parotid flow rate at this time.

关 键 词：放射治疗 腮腺 小型猪 唾液流率 

分 类 号：R818.7[医药卫生—放射医学]