骨髓基质细胞体外培养的生物学特性和成骨能力初步鉴定  

Preliminary identifiation and characterization of in vitro cultured bone marrow stromal cells for their bioactivity and osteogenic potential

作　　者：涂小丽[1] 刘宏伟[2] 李才良[3] 任肖华[4] 

机构地区：[1]南方医科大学珠江医院口腔科,广东广州510282 [2]南方医科大学南方医院口腔科,广东广州510515 [3]武警广东总队医院口腔科,广东广州510516 [4]解放军第458医院口腔科,广东广州510293

出　　处：《南方医科大学学报》2006年第1期111-113,110,共4页Journal of Southern Medical University

基　　金：全军"十五"重点指令性课题(01L075);广东省科技计划项目(C30704)

摘　　要：目的体外分离培养狗的骨髓基质细胞(BMSCs),诱导其向成骨细胞分化,初步鉴定其成骨潜能和生物学特性。方法抽取毕格犬髂骨骨髓体外分离培养获得BMSCs,DMEM、新生牛血清培养基进行原代培养,部分传代细胞以含10 mmol／L地塞米松、50μg／ml抗坏血酸和10 mmol／L β-甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化。倒置相差显微镜进行细胞形态学和细胞生长增殖观察,Von Kossa法染色检测体外矿化结节形成,细胞碱性磷酸酶染色检测BMSCs 的成骨活性。结果体外分离培养的BMSCs经条件培养基诱导后表现出明显的成骨活性,体外矿化(骨样)结节的Von Kossa染色阳性;传代BMSCs碱性磷酸酶染色阳性。结论体外分离培养的BMSCs中含有骨源性前体细胞,传代细胞具有较强的成骨潜能。Objective To induce the differentiation of bone marrow stromall cells （BMSCs） isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs. Methods Primary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with yon Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity. Result BMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results. Conclusion BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.

关 键 词：骨髓基质细胞 生物学特性 成骨潜能 细胞培养 

分 类 号：Q813.11[生物学—生物工程]